Protective role of new nanocomposite natural product (basic nano-curcumin) against breast carcinoma | ||||
| Benha Veterinary Medical Journal | ||||
| Article 13, Volume 33, Issue 2, December 2017, Page 107-116 PDF (528.15 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2017.30006 | ||||
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| Authors | ||||
| Omayma A.R. Abou Zaid1; Fatma A. Ahmed2; Abdel Fatah M. Badwi3; Naglaa M.H. Ibrahim4 | ||||
| 1Clinical Biochemistry Department - Faculty of Veterinary Medicine- Benha University | ||||
| 2Medical and Aromatic Plants Department- Desert Research Center | ||||
| 3Applied Chemistry Department- Egyptian Petroleum Research Institute. | ||||
| 4Medical and Aromatic Plants Department- Desert Research Center. | ||||
| Abstract | ||||
| Present study focused on evaluation of the effect of national nancomposite product on serum biochemical profile and programmed cell death (apoptosis) by using Annexin V-FITC and PI test of breast carcinoma female rats. Basic nanocurcumin was tested for its protective effect on breast cancer female rats (n= 30) divided in two main groups (NTBR) and (TBR) which in turn classified into (TBR- Control Positive and TBR- basic nano- curcumin protective groups). Basic nano-curcumin was formulated and tested for both IC50 (IC50 = 1.0 μg) and LD5o (35 mg/kg by wt.) by using in vitro and in vivo techniques, respectively. Breast cells apoptosis was determined by Annexin V-FITC, which is a fluorescent probe binds to phosphotidylserine (PS) in a Ca 2+ dependent pathway. Breast Cells were stained with Annexin V and PI. The apoptotic cells [Annexin (+) and PI (-)] were differentiated from necrotic cells [Annexin (-) and PI (+)] using flow cytometry technique. The anti-proliferative effect of basic nano-curcumin appeared much earlier due to its nano form. Also, Liver [ALT (40U/l), AST (85 U/l)] and kidney [BUN (19 mg/dl), Creatinine (0.5 mg/dl)] functions were decreased within normal ranges | ||||
| Keywords | ||||
| Basic nano-curcumin; Annexin; BUN; creatinine; AST; ALT | ||||
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