Association between CD3 as a Sensitive T Cells Lineage Marker and NAFLD | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 66, Issue 13, December 2023, Page 2141-2147 PDF (1.25 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2023.173576.7172 | ||||
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| Authors | ||||
Halla Mohamed Ragab  1; Nabila Abd El Maksoud2; Mona A. Amin3; Abdelkarim Kamel4; May Fawzi5; Wafaa Abd Elaziz6
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| 1Biochemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Center, Dokki, Giza Egypt | ||||
| 2Department of Biochemistry, National Research Center, Dokki, Giza, Egypt | ||||
| 3Department of Internal Medicine, Kasr Al-Aini, Cairo University, Egypt | ||||
| 4Medical Biochemistry, Faculty of Medicine, Cairo University | ||||
| 5Department of Internal Medicine, Kasr Al-Ainy , Cairo University, Egypt | ||||
| 6Department of Biochemistry, Biotechnology Research Institute, National Research Center, Dokki, Giza, Egypt | ||||
| Abstract | ||||
| CD3 antigen represents the most specific as well the most sensitive T cells lineage marker, including NK and NKT. Aim and methods: our study was to evaluate CD-3 in nonalcoholic steatohepatitis patients as a diagnostic and prognostic marker in such patients. Serum samples were collected from 41 NAFLD patients, in addition to 14 healthy subjects. They were recruited from the internal medicine department - Cairo University. All patients and controls were subjected to clinical assessment, abdominal ultrasound examination, laboratory assessment including liver function and enzymes, kidney function, and lipid profile. Fib-4 and NAFLD fibrosis score were calculated for all patients. Also, serum levels of CD-3 were detected by ELISA technique. Results: The NAFLD patients group was divided into three groups: fifteen obese patients, fourteen diabetic patients, twelve obese patients with T2DM. BMI, HbA1C, and cholesterol levels were significantly elevated in the NAFLD cases compared with healthy controls (p<0.05). The mean serum level of CD3 was 64.53±84 ng/ml in NAFLD cases vs. 4.76±2.94 ng/ml in controls. Overall, there was a statistically significant increase in serum CD3 levels in NAFLD cases (p<0.001). Additionally, serum level of CD3 was significantly higher in the diabetic non-obese subjects and obese non-diabetic subjects when compared with the lean subjects (89.4±113.53 ng/ml and 60.91±73.76 vs. 4.76±2.94 ng/ml, P = 0. 003& 0.05; respectively. Conclusion: Serum level of CD3 can be used as a prognostic marker in cases with NAFLD as they increase with the progression of the disease and is correlated with fibrosis stage. | ||||
| Keywords | ||||
| NAFLD - CD3; NAFLD fibrosis score; Fib-4 | ||||
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