Isolation and Structure Elucidation of Compounds from Coccinia grandis Leaves Extract
Al-Madhagy, S., Mostafa, N., Youssef, F., Awad, G., Eldahshan, O., Singab, A. (2019). Isolation and Structure Elucidation of Compounds from Coccinia grandis Leaves Extract. EKB Journal Management System, 62(10), 1869-1877. doi: 10.21608/ejchem.2019.10925.1700
Somaia. A. Al-Madhagy; Nada M. Mostafa; Fadia S. Youssef; Ghada. E. A. Awad; Omayma A. Eldahshan; Abdel Nasser B. Singab. "Isolation and Structure Elucidation of Compounds from Coccinia grandis Leaves Extract". EKB Journal Management System, 62, 10, 2019, 1869-1877. doi: 10.21608/ejchem.2019.10925.1700
Al-Madhagy, S., Mostafa, N., Youssef, F., Awad, G., Eldahshan, O., Singab, A. (2019). 'Isolation and Structure Elucidation of Compounds from Coccinia grandis Leaves Extract', EKB Journal Management System, 62(10), pp. 1869-1877. doi: 10.21608/ejchem.2019.10925.1700
Al-Madhagy, S., Mostafa, N., Youssef, F., Awad, G., Eldahshan, O., Singab, A. Isolation and Structure Elucidation of Compounds from Coccinia grandis Leaves Extract. EKB Journal Management System, 2019; 62(10): 1869-1877. doi: 10.21608/ejchem.2019.10925.1700

Isolation and Structure Elucidation of Compounds from Coccinia grandis Leaves Extract

Egyptian Journal of Chemistry
Article 8, Volume 62, Issue 10, October 2019, Page 1869-1877  XML PDF (1.44 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2019.10925.1700
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Authors
Somaia. A. Al-Madhagy1, 2; Nada M. Mostafa1; Fadia S. Youssef1; Ghada. E. A. Awad3; Omayma A. Eldahshan email 1; Abdel Nasser B. Singab1
1Pharmacognosy Department, Faculty of Pharmacy, Ain Shams University. Cario, Egypt,
2Pharmacognosy Department, Faculty of Pharmacy, Sana'a University. Sana'a, Yemen
3Chemistry of Natural and Microbial Products Department. Pharmaceutical and Drug industries research Division. National Research Centre. Giza, Egypt
Abstract
In this study, preliminary phytochemical screening of dried leaves of Coccinia grandis showed presence of triterpenoids, phenolics, flavonoids, saponins and sterols. As well as, five phenolic compounds namely; ferulic acid (1), methyl caffeate (2), ligstroside(3), trans-p- coumaric acid (4) and kaempferol-3-O-β-D-glucoside (5) were isolated from selected fraction of the methanol extract of Coccinia grandis leaves. The structures of the isolated compounds were identified using different NMR spectroscopic techniques (1H, 13C NMR, COSY, HSQC, HMBC) and mass spectrometry. Compounds (1), (2), (4) and (5) were isolated for the first time from Coccinia grandis species while compound (3) was isolated for the first time from Cucurbitaceae family. Moreover, the anti-microbial activity of the selected fraction of the methanol extract of Coccinia grandis leaves was tested against a panel of Gram-positive, Gram-negative bacteria and yeast. A considered activity was recorded against Bacillus cereus (inhibition zone of 20 mm with MIC 1mg/ml).
Keywords
Coccinia grandis; Phenolics; Flavonoids; NMR; MS; Anti-microbial activity
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