TIP-1 is a Degradation Target of HPV-16 E6
elsherbeny, M., thomas, M., Massimi, P., Banks, L. (2023). TIP-1 is a Degradation Target of HPV-16 E6. EKB Journal Management System, 7(2), 47-55. doi: 10.21608/jcbr.2023.186125.1289
mona elhelaly elsherbeny; miranda thomas; paola Massimi; Lawrence Banks. "TIP-1 is a Degradation Target of HPV-16 E6". EKB Journal Management System, 7, 2, 2023, 47-55. doi: 10.21608/jcbr.2023.186125.1289
elsherbeny, M., thomas, M., Massimi, P., Banks, L. (2023). 'TIP-1 is a Degradation Target of HPV-16 E6', EKB Journal Management System, 7(2), pp. 47-55. doi: 10.21608/jcbr.2023.186125.1289
elsherbeny, M., thomas, M., Massimi, P., Banks, L. TIP-1 is a Degradation Target of HPV-16 E6. EKB Journal Management System, 2023; 7(2): 47-55. doi: 10.21608/jcbr.2023.186125.1289

TIP-1 is a Degradation Target of HPV-16 E6

Egyptian Journal of Cancer and Biomedical Research
Volume 7, Issue 2, June 2023, Page 47-55  XML PDF (4.65 MB)
Document Type: Original Article
DOI: 10.21608/jcbr.2023.186125.1289
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Authors
mona elhelaly elsherbeny email orcid 1; miranda thomas2; paola Massimi2; Lawrence Banks2
1medical biochemistry and molecular biology, faculty of medicine , mansoura university,mansoura egypt
2Tumor Virology Group, I.C.G.E.B., AREA Science Park, Padriciano 99, Trieste, Italy
Abstract
Background: The high risk Human Papilloma virus E6 oncoprotein plays an essential role in the development and maintenance of malignancy through its ability to target several PDZ domain containing proteins one of them is a small PDZ protein called Tax-interacting protein 1 (TIP-1). Tip-1 is known to affect the cancer pathway through regulation of Rho and wnt/β-catenin signaling pathways. It also regulates many cellular processes through its interaction with several cellular proteins, including the tax protein, LIN7,MAGI‐1, PlexinD1 , AHDC1, L-glutaminase and Kir2.3. Methods: GST pull down assays , In vitro degradation assays, cell culture and co-transfectio, Immunofluorescent assays and western blot were used to analyse cellular expression levels of TIP-1. Result: TIP-1 was shown to be degraded when co-transfected with E6s from three different cancer-causing HPV types (HPV-16, HPV-18, and HPV-33E6) co-transfection was repeated in the presence of CBZ and we shown by western blot the appearance of Tip-1 again . Also , the co-transfection was repeated in the absence of E6AP and was shown by western blot the appearance of Tip-1 again. Immunofluorescent assay have shown an increase of Tip-1expression in presence of CBZ. Conclusion: Tip-1 is degraded by three different cancer-causing HPV types (HPV-16, HPV-18, and HPV-33E6) This degradation also appears to be dependent on the presence of the E6-AP (UBE3A) protein-ubiquitin ligase. This suggests that TIP-1 is a common target of high-risk HPV types.
Keywords
TIP-1; HPVE6; E6-AP; Human cancer cervix
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