Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1
Morsy, M., Abdelwahab, O., Ibrahim, F., Hassan, H., Alabassery, N. (2023). Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1. EKB Journal Management System, 11(1), 12-29. doi: 10.21608/esctj.2022.179739.1023
Manal Mohammad Morsy; Ola Ali Abdelwahab; Fatma Ibrahim; Heba Ahmed Hassan; Nadia Alabassery. "Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1". EKB Journal Management System, 11, 1, 2023, 12-29. doi: 10.21608/esctj.2022.179739.1023
Morsy, M., Abdelwahab, O., Ibrahim, F., Hassan, H., Alabassery, N. (2023). 'Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1', EKB Journal Management System, 11(1), pp. 12-29. doi: 10.21608/esctj.2022.179739.1023
Morsy, M., Abdelwahab, O., Ibrahim, F., Hassan, H., Alabassery, N. Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1. EKB Journal Management System, 2023; 11(1): 12-29. doi: 10.21608/esctj.2022.179739.1023

Histological and immunohistochemical study on the possible ameliorating effects of antox on the parotid gland of rats with Streptozotocin- Induced Diabetes Mellitus Type 1

Egyptian Society of Clinical Toxicology Journal
Volume 11, Issue 1, June 2023, Page 12-29  XML PDF (1.77 MB)
Document Type: Original Article
DOI: 10.21608/esctj.2022.179739.1023
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Authors
Manal Mohammad Morsy1; Ola Ali Abdelwahab email orcid 2; Fatma Ibrahim3; Heba Ahmed Hassan4; Nadia Alabassery2
1Human Anatomy & Embryology department, Faculty of Medicine, Zagazig University, Egypt.
2Anatomy and embryology department, Faculty of medicine, Zagazig University
3Forensic medicine and clinical toxicology department, Faculty of Medicine, Zagazig University, Egypt
4Clinical pharmacology departments, Faculty of Medicine, Zagazig University, Egypt.
Abstract
Background: Hyperglycemia associated with the diabetes mellitus (DM) commonly results in structural abnormalities and hyposalivation in the parotid glands. An antioxidant action in a potent multivitamin called Antox (ANX) aids in reducing oxidative stress. Aim of study: The current study examined the role of ANX in preventing complications from diabetes in the parotid gland, as well as its potential mechanisms. Material and methods: 36 rats were randomly divided into six groups; each group contained six rats .Group 1 (Negative (-ve) control): the rats received only regular food and water.Group 2 (vehicle group): rats received a single i.p. dose of citrate buffer as a vehicle. Group 3: rats were given ANX by oral gavage with a polyethylene canula 0.5 mm in a volume not to exceed 0.3 ml/100 gm at a dose of 10 mg/kg/day. Group 4 (Diabetic/ DM): rats received a single i.p. dose of 50 mg/kg of STZ dissolved in citrate buffer. Group 5 (DM+ Insulin): rats received a single i.p. dose of 50 mg/kg of STZ dissolved in citrate buffer and insulin (Mixtard 30/70; Novo Nordisk) 1 U/100 gm once daily subcutaneous (S.C.). Group 6 (DM+ Insulin + ANX): rats received a single i.p. dose of 50 mg/kg of STZ and insulin 1 U/100 gm /day/S.C. then received ANX orally in a dose of 10 mg/kg/day. All medications were given for 4 weeks. Results: Remarkably, co-administration of ANX with insulin significantly reduced (MDA) levels and increased expression of (8-OHdG) while enhancing the activities of (SOD), (GPX), and (TAC).
Keywords
Diabetes mellites; oxidative stress; inflammation; apoptosis
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