Morphometric changes among testis of autoimmune mouse model and healthy strains
Shouman, Z. (2023). Morphometric changes among testis of autoimmune mouse model and healthy strains. EKB Journal Management System, 24(1), 10-13. doi: 10.21608/mvmj.2023.301599
Zeinab Shouman. "Morphometric changes among testis of autoimmune mouse model and healthy strains". EKB Journal Management System, 24, 1, 2023, 10-13. doi: 10.21608/mvmj.2023.301599
Shouman, Z. (2023). 'Morphometric changes among testis of autoimmune mouse model and healthy strains', EKB Journal Management System, 24(1), pp. 10-13. doi: 10.21608/mvmj.2023.301599
Shouman, Z. Morphometric changes among testis of autoimmune mouse model and healthy strains. EKB Journal Management System, 2023; 24(1): 10-13. doi: 10.21608/mvmj.2023.301599

Morphometric changes among testis of autoimmune mouse model and healthy strains

Mansoura Veterinary Medical Journal
Volume 24, Issue 1, March 2023, Page 10-13  XML PDF (428.58 K)
Document Type: Original Articles
DOI: 10.21608/mvmj.2023.301599
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Author
Zeinab Shouman
Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt
Abstract
Objective: to compare morphometrically among testis of the healthy strains (C57BL/6N and MRL/MpJ) and autoimmune mouse model (MRL/MpJ-Faslpr) revealing how the autoimmune disease affect cells lining the seminiferous tubules which in turn cause infertility. 
Design: Descriptive study.
Sample: The testis of both healthy strains (C57BL/6N and MRL/MpJ) and autoimmune mouse model (MRL/MpJ-Faslpr) at early (3 months) and late (6 months) age were used (4 mice from each strain and age).
Procedures: The testis were removed, fixed and immunostained by PAX7, CKIT, GATA4 as well as Hoechest to reveal the morphometric changes among them at early (3 months) and late (6 months) age.
Results: Both MRL/MpJ and MRL/MpJ-Faslpr testis at 6 months showed significant decrease of GATA4 positive cells while their parent strain (C57BL/6N) testis showed significant increase. Furthermore, MRL/MpJ and MRL/MpJ-Faslpr 6 months testis revealed a significant increase in sertoli cell index when counted for CKIT positive cells and significant decrease in their parent strain at the same age, while all studied strains showed a non-significant decrease in sertoli cell index when counted for PAX7 positive cells at 6 months of age.
Conclusion and clinical relevance: We concluded that the normal age-related changes including decrease of spermatogenic cells (PAX7 and CKIT positive cells) and increase of sertoli cells (GATA4 positive cells) occurred in the parent strain (C57BL/6N) while deviated than normal at late age of both MRL/MpJ and MRL/MpJ-Faslpr.
Keywords
Autoimmune disease; MRL/MpJ-Faslpr; sertoli cell; testis
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