Protective Effect of Curcumin and Cerium Oxide Nanoparticles on Carboplatin Induced Myelotoxicity and Hepatotoxicity in Adult Male Wistar Rats

Amer, Marwa; Abdel Moawed, Dena; Sameh, Reham; Agaga, Rasha Ahmed; Elwany, Nisreen; Abdelhamid, Amira Mohamed

doi:10.21608/zjfm.2023.194806.1142

	Protective Effect of Curcumin and Cerium Oxide Nanoparticles on Carboplatin Induced Myelotoxicity and Hepatotoxicity in Adult Male Wistar Rats
Zagazig Journal of Forensic Medicine
Article 4, Volume 21, Issue 2, July 2023, Page 55-83 PDF (1.43 MB)
Document Type: Original Article
DOI: 10.21608/zjfm.2023.194806.1142
View on SCiNiTO
Authors
Marwa Amer ¹; Dena Abdel Moawed ²; Reham Sameh ³; Rasha Ahmed Agaga⁴; Nisreen Elwany ⁵; Amira Mohamed Abdelhamid ⁶
¹Forensic Medicine and Clinical Toxicology, Faculty of Medicine , Zagazig University
²Forensic medicine and toxicology department Faculty of medicine Zagazig university
³Pathology Department, Faculty of medicine, Zagazig University
⁴Human anatomy and embryology, faculty of medicine, Zagazig university
⁵Clinical pharmacy department, faculty of medicine, Zagazig university
⁶Clinical pharmacology Department, Faculty of medicine, Zagazig University
Abstract
Background: Carboplatin is a chemotherapeutic agent used in many types of cancers. Carboplatin adversely affects multiple organs like the bone marrow, liver, gastrointestinal tract, and kidney. Aim of the study: The aim of this work is to evaluate the potential protective role of curcumin and cerium oxide nanoparticles (CONPs) on carboplatin induced myelotoxicity and hepatotoxicity. Methods: Forty-eight adult male Wistar rats were classified into seven groups; I. Control (Subdivided into negative and positive control groups), *II.* Curcumin-treated, *III.* CONPs-treated, *IV.* Carboplatin intoxicated, V. Curcumin-treated carboplatin intoxicated, *VI.* CONPs-treated carboplatin intoxicated & *VII.* Curcumin & CONPs-treated carboplatin intoxicated groups. Curcumin was administered orally, once daily for 4 consecutive weeks. CONPs, suspended in saline, and carboplatin were administered intraperitoneally once a week for 4 consecutive weeks.After 4 weeks, blood samples were collected after anesthesia of rats. The animals were then sacrificed, with bone marrow samples & livers collected for biochemical, histopathological and immunohistochemical studies. Results: Carboplatin treatment decreased blood cells count and elevated liver enzymes and bone marrow & hepatic malondialdehyde (MDA). It decreased GSH levels and causes DNA damage. Carboplatin induced hypocellularity in bone marrow samples and showed Strong 8-OHdG immunoreactivity. It also induced septal fibrosis with architectural distortion in liver tissue. Administration of either curcumin or CONPs ameliorated these toxic effects of carboplatin. Meanwhile the concurrent administration of curcumin and CONPs revealed much better improvement than each of them used alone. Conclusions: Combined administration of curcumin and CONPs ameliorated carboplatin-induced myelotoxicity and hepatotoxicity through antioxidant mechanism.
Keywords
Curcumin; Cerium oxide; Nanoparticles; Carboplatin; Myelotoxicity; Hepatotoxicity


Statistics Article View: 247 PDF Download: 226