Physiological responses of Greater wax moth (Galleria mellonella) to inoculation with some strains of endophytic fungi

Fekry, Doaa ElSayed; ElSheikh, Ali Abd ElAziz; Mohamed, Gamal El-didamony; Ismaiel, Ahmed Abd ElRahman; El-Gendy, Rehab Mahmoud

doi:10.21608/bfszu.2022.174940.1207

	Physiological responses of Greater wax moth (Galleria mellonella) to inoculation with some strains of endophytic fungi
Bulletin of Faculty of Science, Zagazig University
Article 12, Volume 2023, Issue 2, June 2023, Page 121-128 PDF (900.05 K)
Document Type: Original Article
DOI: 10.21608/bfszu.2022.174940.1207
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Authors
Doaa ElSayed Fekry ¹; Ali Abd ElAziz ElSheikh²; Gamal El-didamony Mohamed³; Ahmed Abd ElRahman Ismaiel⁴; Rehab Mahmoud El-Gendy⁵
¹Al Thrir St - Abou Keebr - Sharkia
²Plant protection research institute, Agriculture Research Centre
³Department of Microbiology and Botany, Faculty of Science, Zagazig University, 44519, Zagazig, Egypt.
⁴Botany department, Faculty of science, Zagazig university, Zagazig, Egypt
⁵Plant Protection Institute, Zagazig, Egypt
Abstract
This study was carried out to investigate the effect of endophytic fungi on physiological responses of Galleria mellonella (G. mellonella). The analyses of some parameters have been recorded as influenced by the filtrate of A. nidulans, A. flavus and A. niger. After 3 days, A. flavus AUMC 13942, A. niger AUMC 13944 strain produced the highest GOT activity of up to 2.16 and 1.29 µg pyruvate\ g. b. wt\ min, respectively. The increase in incubation period caused a notable decrease in GOT activity of G. mellonella larvae with the treated fungal strains. A. flavus AUMC 13942 causes a significant decrease (P<0.05) in GOT enzyme activity after 5 days then causes disturbance in its values to the end of experiment, these values are 1.25 and 0.13 µg/ g.b.wt after 7 and 9 days respectively. A. nidulans, A. niger and A. flavus cause the highest increase in the enzyme activity after 3 days and their values are 42.27, 21.91 and 8.81 µg/ g.b.wt respectively, then decrease again until the end of experiment. It can be seen that, the highest increase recorded after treatment of LC50 of A. nidulans after 3 days, while the lowest value of the enzyme recorded 0.11 after treatment with LC50 of A. niger after 9 days of treatment. Sub lethal concentrations of A. nidulans AUMC 13941 produce the highest increase in ACP enzyme activity at the 3rd day with value (106.43 µg/ g).
Keywords
Galleria mellonella; endophytic fungi; Physiological


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