Surveillance for SARS-CoV-2 Variants of Concern and Initial Detection of Omicron using RT-PCR in the Kurdistan region of Iraq | ||||
| SVU-International Journal of Medical Sciences | ||||
| Article 39, Volume 6, Issue 2, July 2023, Page 408-415 PDF (286.86 K) | ||||
| Document Type: Original research articles | ||||
| DOI: 10.21608/svuijm.2023.189979.1510 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Dlshad Abdullah Hasan1; Sazan Qadir Maulud2; Sharmeen Qadr Faqi1; Rzgar Farooq Rashid3; Jivan Qasim Ahmed  4; Karzan Abdulmuhsin Mohammad5
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| 1Molecular Diagnostic Department, Central Public Health Laboratory-Erbil, Kurdistan Region, Iraq. | ||||
| 2Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq. | ||||
| 3Department of Medical Laboratory Science, College of Science, Knowledge University, Erbil 44001, Iraq | ||||
| 4Department of Pathology and Microbiology, University of Duhok, Kurdistan Region, Iraq. | ||||
| 5General Directorate of Scientific Research Center, Salahaddin University-Erbil, Erbil, Iraq. | ||||
| Abstract | ||||
| Background:Omicron (B.1.1.529), a novel SARS-CoV-2 variant (VOC), is a highly diverse variant with many mutations. Immune evasion is possible, as is increased transmissibility within the populations. The RT-qPCR method may be effectively utilised for variant surveillance. This is to rule in or rule out significant variants quickly. Objectives: As a result, the goal of this study was to track the prevalence of the SARS-CoV-2 Omicron variant in the local community using the SGTF test in conjunction with the SARS-CoV-2 S-gene mutations RT-PCR assays. Patients and methods: The study included 255 SARS-CoV-2 positive specimens collected in Erbil central public health laboratory between January 1 to February 6, 2022, for routine testing purposes. The SARS-CoV-2 variant profiling was performed on extracted RNA using PowerChek SARS-CoV-2 S-gene Mutation Detection Kit Ver.3.0 plus S-gene Target Failure (SGTF) of the TaqPath™ COVID-19 CE-IVD RT-PCR Kit. Results: The samples were surveyed, resulting in a positivity rate of (86.6%) for Omicron BA.1, (3.1%) Omicron BA.2, (1.7%) Delta variant (B.1.617.2), and (8.6%) were inconclusive variants. Among Omicron COVID-19 cases, 89 (38.5%) were fully vaccinated, and 4 (1.7%) received full vaccination plus a booster dose. Nevertheless, 16 (7%) of the confirmed Omicron COVID-19 cases had a documented previous SARS-CoV-2 infection. Conclusion:The SARS-CoV-2 S-gene mutations RT-PCR assay is a cost-effective and fast method for the surveillance of SARS-CoV-2 variants of concern. Currently, Omicron BA1 is the predominant SARS-CoV-2 variant in the Kurdistan region/Iraq, and the emergence of the Omicron BA2 variant is of high concern. | ||||
| Keywords | ||||
| SARS-CoV-2; Omicron; Variants of concern | ||||
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