Histological and Immuno-histochemical Responses of Zinc oxide Nanoparticles and Ethanolic Extract of Senecio glaucus L. With the Potential Anti- apoptotic Effect of Gallic Acid in Lungs’ Rats | ||||
| Zagazig Journal of Forensic Medicine and Toxicology | ||||
| Article 6, Volume 21, Issue 2, July 2023, Page 98-114 PDF (834.78 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zjfm.2023.211814.1150 | ||||
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| Authors | ||||
Nabil Moustafa 1; Ali EL Sheikh 2; Nouran EL-Shafey  3
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| 1Organic Division, Chemistry Department, Faculty of Science, Zagazig University, Egypt. | ||||
| 2Department of Pest Physiology Research, Plant Protection Research Institute, Agriculture Research Center, Egypt. | ||||
| 3Department of Pest Physiology Research, Plant Protection Research Institute, Agriculture Research Center, Egypt - Biochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, Egypt | ||||
| Abstract | ||||
| BBackground: Zinc oxide nanoparticles (ZnO NPs) are used in different fields. Mureer or Seneico glaucus L. plant (SP) is the most important plants spreading in the deserts that animals feed on it. ZnO NPs and SP can be used separately or combined as supplements of alternative insecticides in the agriculture program for crop protection. Gallic acid (GA) is an important antioxidant that found in many types of fruits. Objectives: The purpose of this study was to evaluate the effects of either single or dual treatments of ZnO NPs and SP on lung tissue through histological and immunohistochemical investigations, and to estimate the prospective role of GA in rats for 30 days. Material & methods: Rats were allocated into eight groups with orally administrated: Control, GA (100 mg/kg), ZnO NPs (150 mg/kg), SP (400 mg/kg), GA+ZnO NPs (100,150 mg/kg), GA+SP (100,400 mg/kg), ZnO NPs+SP (150,400 mg/kg), and GA+ZnO NPs+SP (100,150,400 mg/kg). Results: Results indicated that either single or dual treatments of ZnO NPs and SP induced dense thickening in the alveolar wall, interstitial inflammation, and induced an overexpression of caspase-3 immune response. Further, the effect of dual treatment was more obvious than the influence of alone one. Inversely, GA ameliorates these undesirable changes and down-expressed of caspase-3 immune response. Conclusion: Our findings revealed that ZnO NPs and SP are pulmonary-toxic agents and GA might be cyto-protective and anti-apoptotic agent against the induced pulmonic-toxicity. | ||||
| Keywords | ||||
| Zinc oxide nanoparticles (ZnO NPs); Mureer or Senecio glaucus L. plant (SP); Gallic acid (GA); Lung tissue; Caspase-3 protein | ||||
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