Insecticidal and phytochemical investigation of Catharanthus roseus L. extracts to Spodoptera littoralis (Boisd.)
Mostafa, M., Abdelmonem, A., El-Ablack, F., Abdel-Mogib, M., Ayyad, S. (2023). Insecticidal and phytochemical investigation of Catharanthus roseus L. extracts to Spodoptera littoralis (Boisd.). EKB Journal Management System, 13(1), 39-47. doi: 10.21608/sjdfs.2023.212771.1111
Mohamed El-Hosieny Mostafa; Ahmed Abdelmonem; Fawzia Zakaria El-Ablack; Mamdouh Abdel-Mogib; Seif-Eldin Nasr Ayyad. "Insecticidal and phytochemical investigation of Catharanthus roseus L. extracts to Spodoptera littoralis (Boisd.)". EKB Journal Management System, 13, 1, 2023, 39-47. doi: 10.21608/sjdfs.2023.212771.1111
Mostafa, M., Abdelmonem, A., El-Ablack, F., Abdel-Mogib, M., Ayyad, S. (2023). 'Insecticidal and phytochemical investigation of Catharanthus roseus L. extracts to Spodoptera littoralis (Boisd.)', EKB Journal Management System, 13(1), pp. 39-47. doi: 10.21608/sjdfs.2023.212771.1111
Mostafa, M., Abdelmonem, A., El-Ablack, F., Abdel-Mogib, M., Ayyad, S. Insecticidal and phytochemical investigation of Catharanthus roseus L. extracts to Spodoptera littoralis (Boisd.). EKB Journal Management System, 2023; 13(1): 39-47. doi: 10.21608/sjdfs.2023.212771.1111

Insecticidal and phytochemical investigation of Catharanthus roseus L. extracts to Spodoptera littoralis (Boisd.)

Scientific Journal for Damietta Faculty of Science
Volume 13, Issue 1, June 2023, Page 39-47  XML PDF (361.6 K)
Document Type: Original articles
DOI: 10.21608/sjdfs.2023.212771.1111
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Authors
Mohamed El-Hosieny Mostafa email orcid 1; Ahmed Abdelmonem2; Fawzia Zakaria El-Ablack3; Mamdouh Abdel-Mogib4; Seif-Eldin Nasr Ayyad5
1Plant Protection Research Institute Agriculture Research Center
2Plant protection research institute
3Chemistry Department, Damietta Faculty of Science
4Chemistry Department, Faculty of Science, Mansoura University
5Chemistry Department, Faculty of Science Mansoura University, Mansoura
Abstract
Chromatographic separation of Catharanthus roseus yielded six fractions. Of the sex fractions, the alkaloid and butanol fractions were the most effective fraction against Spodoptera littoralis (Boisd.) larvae in their second instar after seven days of exposure using leaf dipping technique with LC50 value 234.58 and 481.09 ppm, respectively. Toxicological effect guided isolation and elucidation of the most toxic fraction and yielded three monoterpenoid indole alkaloids, vindoline (1), vindorosin (2) and ajmalicine (3) using extensive chromatographic and spectroscopic techniques. The effect of alkaloid and butanol fractions on biochemical aspects including transaminases enzymes (AST and ALT), acelylcolinesterase and chitinase for S. littoralis, 2 nd instar larvae were evaluated. A significant activation on AST and ALT activities were obtained after treatment with LC50 of alkaloid and butanol fractions also, both fractions might be used as AchE inhibitors and suggested an ability in biodegradation of chitin. The sublethal effect of both alkaloid and butanol fractions on the biological aspects of S. littoralis immature and adult stages were studied in details.
Keywords
Catharanthus roseus,Spodoptera littoralis; alkaloids; biological and biochemical responses
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