EVALUATION OF THE ANTICARCINOGENIC EFFECT OF TAK-242, A TLR4 INHIBITOR, IN ORAL SQUAMOUS CELL CARCINOMA (IN VITRO STUDY) | ||||
Alexandria Dental Journal | ||||
Article 3, Volume 49, Issue 1, April 2024, Page 17-21 PDF (635.66 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/adjalexu.2023.198402.1357 | ||||
View on SCiNiTO | ||||
Authors | ||||
Heba Allah Mohamed Mostafa 1; Sahar Mohamed Elsheikh2; Radwa Ali Mehanna3; Enas Magdi Omar4 | ||||
1Oral pathology department, Faculty of dentistry, Alexandria University, Alexandria, Egypt | ||||
2Oral Pathology department, faculty of dentistry, Alexandria university,Egypt. | ||||
3Department of Medical Physiology, Faculty of Medicine, Alexandria University, Egypt | ||||
4Oral Pathology department, faculty of dentistry, Alexandria university,Alexandria, Egypt. | ||||
Abstract | ||||
Introduction: Cancer immunotherapy has been recently presented as one of the most efficient treatment approaches for many types of cancer. Toll-like receptor 4 (TLR4) upregulation is involved in the proliferation, migration, and metastasis of many tumors. TAK-242 (also known as Resatorvid) is a TLR4 antagonist which specifically binds to the intracellular domain of the receptor and inhibits all TLR4 interplays with its downstream signaling molecules suggesting a new promising anticarcinogenic strategy. Objectives: The present research aimed to estimate the antitumorigenic effects of TAK-242 on oral squamous cell carcinoma-4 (OSCC-4) cell line. Methodology: Verification of TLR4 expression in OSCC-4 cells was established using immunocytochemistry to proof the presence of the studied receptor in the cell line. Next, the cytotoxic effect of TAK-242 was evaluated using MTT assay. The sublethal IC50 dose was calculated then apoptotic induction ability of the drug was assessed using Annexin V/PI assay. Results: In the present research, it was concluded that TLR4 was significantly expressed in OSCC-4 cells. In addition, its inhibitor (TAK-242) halted cell proliferation by inducing apoptosis of OSCC-4 cells. Conclusions: TAK-242 has a significant anticarcinogenic effect on OSCC-4 cells and could be further studied experimentally. | ||||
Keywords | ||||
Innate immunity; TLR4; Oral squamous cell carcinoma; TAK-242 | ||||
Statistics Article View: 145 PDF Download: 171 |
||||