MOLECULAR DETECTION OF METHICILLIN RESISTANCE STAPHYLOCOCCUS AUREUS FROM HORSES IN EGYPT | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 80, Issue 2, December 2020, Page 365-374 PDF (666.88 K) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| Staphylococcus aureus (S. aureus) is a Gram positive organism that serves as an opportunistic pathogen and frequent colonizer of the epithelium causing severe diseases in human and animals. The widespread use of antibiotics both in human and veterinary medicine resulted in the emergence of resistant strains of S. aureus. This resistance is determined by the mecA gene, which encodes the low-affinity penicillin-binding protein (PBP 2). MRSA infection was first considered hospital-associated (HA-MRSA) and community-associated MRSA (CA-MRSA) infections. However, another group emerged known as livestock-associated MRSA (LA-MRSA). The isolation of MRSA from different species, food products and the environment raised concern on the role of animals particularly livestock and wildlife in the epidemiology of MRSA.The spatial distribution of MRSA indicates interspecies transmission and colonization. of different populations. The purpose of this study was to detect the prevalence of MRSA from the nostril and wound of horses. Samples were collected from nostrils and wounds of 92 horses. The isolation and identification of MRSA were carried out on selective media (Mannitol Salt agar and Blood agar) and confirmed using series of biochemical tests. The isolates were also tested for the presence of mecA gene. A high proportion of MRSA (61.2%) MRSA were obtained from the samples.Antibiotic sensitivity tests (AST) demonstrated the multiresistance characteristics of the MRSA isolates | ||||
| Keywords | ||||
| MRSA; PCR; mec-A gene; Horse; Human; antibiotic sensitivity | ||||
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