Evaluation and Comparison of Four Screening Tests against Milk Culture for Detection of Subclinical Mastitis in Lactating Cattle and Buffalo in Egypt

Alkhouly, Ibrahim N.; Moustafa, Abdelmoneim A.; Abou El Roos, Nahla A.; Kandeel, Sahar A.

doi:10.21608/javs.2023.211272.1234

	Evaluation and Comparison of Four Screening Tests against Milk Culture for Detection of Subclinical Mastitis in Lactating Cattle and Buffalo in Egypt
Journal of Applied Veterinary Sciences
Article 8, Volume 8, Issue 3, July 2023, Page 67-74 PDF (657.29 K)
Document Type: Original Article
DOI: 10.21608/javs.2023.211272.1234
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Authors
Ibrahim N. Alkhouly¹; Abdelmoneim A. Moustafa²; Nahla A. Abou El Roos¹; Sahar A. Kandeel ²
¹Animal health Research Institute, Shibin El Koom branch, Agriculture Research center, Egypt
²Department of Animal Medicine (Infectious Diseases), College of Veterinary Medicine, Benha University, Kalyobiya, 13637, Egypt.
Abstract
Subclinical mastitis (SCM) is an asymptomatic worldwide udder infection that results in substantial losses to the dairy industry. Our main objective was to evaluate and compare the clinical performance of 4 commercially available screening tests for diagnosing SCM. Foremilk samples were collected from 428 quarters of 107 apparently healthy lactating cows and buffaloes from El-Menofia governorate from 2020 to 2022. Quarter somatic cell count (SCC) was estimated using the FOSS-BacSomatic^® counter and the California Mastitis Test (CMT), with SCM defined as SCC > 200,000 cells/mL. Milk pH was measured cow-side using an AD11^®pH-meter and BOVIVET^® indicator paper. Bacterial cultures of foremilk samples were used to diagnose SCM as a reference method based on the isolation of the causative pathogens. The tests’ performance was evaluated by calculating test sensitivity (Se), specificity (Sp), and accuracy at the optimal-cut-point for each test. FOSS-BacSomatic^® counter was the best-performing test for diagnosing SCM (Se = 0.967, Sp = 0.943, accuracy = 0.957) at an optimal-cut-point of >200,000 cells/mL. For comparison, CMT is the second best-performing test at an optimal-cut-point of a non-negative score (Se = 0.892, Sp = 0.878, accuracy = 0.887). The test performance of the AD11^®pH-meter and BOVIVET^® indicator paper was fair, however, the AD11^®pH-meter performed better than the BOVIVET^® indicator paper with Se = 0.807, Sp = 0.845, and accuracy = 0.822. We concluded that the FOSS-BacSomatic^® counter and CMT are considered good tests for diagnosing SCM. On the other hand, milk pH doesn’t provide a clinically useful method for diagnosing SCM. However, based on cost, availability and analysis time, there doesn't seem to be a persuasive reason to select the FOSS-BacSomatic^® counter over the traditional CMT to diagnose SCM.
Keywords
California mastitis test; Milk pH; Somatic cell count; Subclinical mastitis


References
ABDEL-GHANY, A. 2011. Parasitological and immunological studies on anisakid parasites. PhD, Zagazig, Egypt. ABDELSALAM, M., ATTIA, M. M., and MAHMOUD, M. A., 2020. Comparative morphomolecular identification and pathological changes associated with Anisakis simplex larvae (Nematoda: Anisakidae) infecting native and imported chub mackerel (Scomber japonicus) in Egypt. Regional Studies in Marine Science, 39, 101469. ABOU-RAHMA, Y., ABDEL-GABER, R., and KAMAL AHMED, A., 2016. First record of Anisakis simplex third-stage larvae (Nematoda, Anisakidae) in European Hake Merluccius merluccius lessepsianus in Egyptian water. J Parasitol Res 2016:9609752. https://doi.org/10.1155/2016/9609752 ADAMS, A. M., MILLER, K. S., WEKELL, M.M., and DONG, F. M., 1999. Survival of Anisakis simplex in microwave-processed arrow tooth flounder. J. Food Prot. 4:403–409. AIBINU, I. E., SMOOKER, P. M., and LOPATA, A. L., 2019. Anisakis Nematodes in Fish and Shellfish- from infection to allergies. International journal for parasitology. Parasites and wildlife, 9, 384–393. https://doi.org/10.1016/j.ijppaw.2019.04.007 AL-ABSAWY, M.A.E.G. 2008. Reproductive biology, spermatogenesis and ultrastructure of the testes of Gadidae fish, Merluccius merluccius (Linnaeus 1758). Egyptian Journal of Aquatic Research. 34 NO. 3, 286-303. AL-ABSAWY, M.A.E.G. 2010. The reproductive biology and the histological and ultrastructural characteristics in ovaries of the female gadidae fish, Merluccius merluccius from the Egyptian Mediterranean water. African Journal of Biotechnology, 9, 2544-2559. ANDALORO, F., ARENA, P., and PRESTIPINO GIARRITTA, S., 1985. Contribution to the knowledge of the age, growth and feeding of hake Merluccius merluccius (L. 1758) in the Sicilian Channel. FAO Fish. Rep. 336, 93–7. ARAFA, W.M., HASSAN, A.H.A., MAHROUS, L.N., ABDEL-GHANY, A.E., and ABOELHADID, S.M., 2019. Occurrence and molecular characterization of zoonotic Anisakis simplex sensu stricto and Anisakis pegreffii larvae in retail-marketed fish. J. Food Saf. 39, 1–7. https://doi.org/10.1111/jfs.12682 AUDICANA, L., AUDICANA, M.T., DE CORRES, L.F., and KENNEDY, M.W., 1997. Cooking and freezing may not protect against allergic reactions to ingested Anisakis simplex antigens in humans. Vet. Rec.,140, 235. BANCROFT, J.D., and Gamble, M., 2008. Theory and practice of histological techniques.6th Edition, Churchill Livingstone, Elsevier, China. BAROT, S., HEINO, M., BRIEN, L., and DIECKMANN, U., 2004. Estimating reaction norms for age and size at maturation when age at first reproduction is unknown. Evolutionary ecology research, 6: 659-678. BOURREE, P., PAUGAM, A., and PETITHORY, J.C., 1995. Anisakidosis: Report of 25 cases and review of the literature. Comp. Immunol. Microbiol. Infect. Dis. 18, 75–84 CARBONARA, P., PORCU, C.; DONNALOIA, M., PESCI, P., SION, L., SPEDICATO, M. T., ZUPA, W., VITALE, F., and FOLLESA, M. C., 2019. The spawning strategy of European hake (Merluccius merluccius, L. 1758) across the Western and Central Mediterranean Sea. Fisheries Research, 219, 105333. doi: https://doi.org/10.1016/j.fishres.2019.105333 CEBALLOS-MENDIOLA, G., VALERO, A., POLO-VICO, R., TEJADA, M., ABATTOUY, N., KARL, H., DE LAS HERAS, C., and MARTÍN-SÁNCHEZ, J., 2010. Genetic variability of Anisakis simplex s.s. parasitizing European hake (Merluccius merluccius) in the Little Sole Bank area in the Northeast Atlantic. Parasitology Research, 107: 1399–1404. https://doi.org/10.1007/s00436-010-2009-5 COSTA, A., CAMMILLERI, G., GRACI, S., BUSCEMI, M.D., VAZZANA, M., PRINCIPATO, D., GIANGROSSO, G., and FERRANTELLI, V., 2016. Survey on the presence of A. simplex s.s. and A. pegreffii hybrid forms in Central-Western Mediterranean Sea. Parasitol. Int. 65, 696–701. DOLORS FERRER-MAZA, JOSEP LLORET, MARTA MUÑOZ, ELISABETH FALIEX, SÍLVIA VILA, and PIERRE SASAL, 2014. Parasitism, condition and reproduction of the European hake (Merluccius merluccius) in the northwestern Mediterranean Sea, ICES Journal of Marine Science, Volume 71, Issue 5, July/August, Pages 1088–1099. https://doi.org/10.1093/icesjms/fst217 EISSA, A.E., ABDELSALAM, M., ATTIA, M.M., ISMAIL, G.A., QORANY, R.A., PRINCE, A., and ABDEL-MONEAM, D.A., 2021. Morphological, molecular and clinical assessment of different Anisakis species infecting horse Mackerel Trachurus trachurus from South Mediterranean. Egyptian Journal of Aquatic Biology and Fisheries, 25(4), 1085-1098.‏ EISSA, A.E., SHOWEHDI, M.L., ISMAIL, M.M., EL-NAAS, A.S., ABU MHARA, A.A., and ABOLGHAIT, S.K., 2018. Identification and prevalence of Anisakis pegreffii and A. pegreffii XA. Simplex (s.s.) hybrid genotype larvae in Atlantic horse Mackerel (Trachurus trachurus) from some North African Mediterranean coasts. Egyptian Journal of Aquatic Research 44; 21–27. FARJALLAH, S., BUSI, M., MAHJOUB, M. O., SLIMANE, B. B., PAGGI, L., SAID, K., and D’AMELIO, S., 2008. Molecular characterization of larval anisakid nematodes from marine fishes off the Moroccan and Mauritanian coasts. Parasitology International, 57: 430–436. FERRER-MAZA, D., LLORET, J., MUÑOZ, M., FALIEX, E., VILA, S., and SASAL, P., 2016. Links between parasitism, energy reserves and fecundity of European anchovy, Engraulis encrasicolus, in the northwestern Mediterranean Sea. Conservation Physiology, 4(1), cov069. GAFRD, 2019. General Authority for Fishery Resources Development. Fish statistics yearbooks. Ministry of Agriculture and Land Reclamation, Cairo, Egypt. GAGLIO, G., BOTTARI, T., RINELLI, P., MARINO, F., MACRI`, D., and GIANNETTO, S., 2011. Prevalence of Clavella stellata (Copepoda: Lernaeopodidae) (Kroyer 1838) in the European hake (Merluccius merluccius) (L. 1758), South Tyrrhenian Sea (Central Mediterranean). Journal of Applied Ichthyology, 27: 136–138. https://doi.org/10.1111/j.1439-0426.2010.01526.x GIARRATANA, F., MUSCOLINO, M., BENINATI, C., GIUFFRIDA, A., and PANEBIANCO, A., 2014. Activity of Thymus vulgaris essential oil against Anisakis larvae. Exp. Parasitol. 142:7–10. https://doi.org/10.1016/j.exppara.2014.03.028 GRABDA, J., and SOLIMAN, I.A.F.M., 1975. Copepods—Parasites of the genus Merluccius from the Atlantic Ocean and Mediterranean Sea. Acta Ichthyologica et Piscatoria, 5: 31–40 HABOUZ, H., RECASENS, L., KIFANI, S., MOUKRIM, A., BOUHAIMI, A., and AYOUBI, S., 2011. Maturity and batch fecundity of the European hake (Merluccius merluccius, Linnaeus, 1758) in the eastern central Atlantic. Scientia Marina, 75. https://doi.org/10.3989/scimar.2011.75n3447 ISHIKURA, H., KIKUCHI, K., NAGASAWA, K., OOIWA, T., TAKAMIYA, H., SATO, N., and SUGANE, K., 1993. Anisakidae and anisakidosis. In: Sun, T. (Ed.), Progress in Clinical Parasitology 8. Springer Verlag, N. Y., pp. 43–102. KAHRAMAN, A., YILDIZ, T., UZER, U., and KARAKULAK, F., 2017. Sexual Maturity and Reproductive Patterns of European Hake, Merluccius merluccius (Linneaus, 1758) (Actinopterygii: Merlucciidae) from the Sea of Marmara, Turkey. MATTIUCCI, S., and NASCETTI, G., 2008. Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and ostparasite co-evolutionary process. Adv. Parasitol. 66, 47–148. MATTIUCCI, S., CIPRIANI, P., WEBB, S.C., PAOLETTI, M., MARCER, F., BELLISARIO, B., GIBSON, D.I., and NASCETTI, G., 2014. Genetic and morphological approaches distinguish the three sibling species of the Anisakis simplex species complex, with a species designation as Anisakis berlandi n. sp. for A. simplex sp. C (Nematoda: Anisakidae). J. Parasitol., 100 (2), 199–214. https://doi.org/10.1645/12-120.1 MATTIUCCI, S., PAGGI, L., NASCETTI, G., ISHIKURA, H., KIKUCHI, K., SATO, N., CIANCHI, R., and BULLINI, L., 1998. Allozyme and morphological identification of Anisakis, Contracaecum and Pseudoterranova from Japanese waters (Nematoda, Ascaridoidea). Syst. Parasitol., 40, 81–92. MCGLADDERY, S.E. 2011. Anisakis simplex (Nematoda: Anisakidae) infection of the musculature and body cavity of Atlantic herring (Clupea harengus harengus). Can J Fish Aquat Sci 34:1312–1317. https://doi.org/10.1139/f86-164. MEHANNA, S.F., HAGGAG, H.M., and AMIN, A.M., 2005. Preliminary evaluation of the status of Port Said Fisheries at the Mediterranean Coast of Egypt. Egypt. J. Aquat. Biol & Fish., 9 (4): 229- 245 pp. MICHELA, C., ROMAIN, F., SABRINA, C., ALBERTO, S., FINN-ARNE, W., and OLIANA, C., 2017. Gonadotropin characterization, localization and expression in the European hake (Merluccius merluccius). Reproduction, 153(2), 123-132. https://doi.org/10.1530/rep-16-0377 MLADINEO, I., ŠIMAT, V., MILETIĆ, J., BECK, R., and POLJAK, V., 2012. Molecular identification and population dynamic of Anisakis pegreffii (Nematoda: Anisakidae Dujardin, 1845) isolated from the European anchovy (Engraulis encrasicolus L.) in the Adriatic Sea. International journal of food microbiology, 157(2), 224-229.‏ MUÑOZ-CARO, T., MACHUCA, A., MORALES, P., VERDUGO, J., REYES, R., GARCÍA,M., RUTAIHWA, L., SCHINDLER, T., POPPERT, S., TAUBERT, A., and HERMOSILLA, C., 2022. Prevalence and molecular identification of zoonotic Anisakis and Pseudoterranova species in fish destined to human consumption in Chile. Parasitol Res 121, 1295–1304. https://doi.org/10.1007/s00436-022-07459-x. OLIVER, P., and MASSUTÍ, E., 1995. Biology and fisheries of western Mediterranean hake (M. merluccius) . In: Alheit, J., Pitcher, T.J. (eds) Hake. Chapman & Hall Fish and Fisheries Series, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1300-7_7. PHILIPS, A., and RAGHEB, E., 2013. Reproductive biology of European hake, Merluccius merluccius (Linnaeus, 1758) in the Egyptian Mediterranean waters. Egyptian Journal of Aquatic Biology and Fisheries, 17(4), 37-47. https://dx.doi.org/10.21608/ejabf.2013.2184 RAIBAUT, A., COMBES, C., and BENOIT, F., 1998. Analysis of the parasitic copepod species richness among Mediterranean fish. Journal of Marine Systems, 15: 185–206. RECASENS, L., CHIERICONI, V., BELCARI, P. J., and MARINA, S., 2008. Spawning pattern and batch fecundity of the European hake (Merluccius merluccius (Linnaeus, 1758)) in the western Mediterranean. 72, 721-732.
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