The Growth and Biochemical Composition of Nannochloropsis oculata Under Influence of Different Phosphorus Sources for Aquaculture Live food | ||||
| Mediterranean Aquaculture Journal | ||||
| Volume 10, Issue 1, June 2023, Page 10-22 PDF (775.92 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/maj.2023.215574.1024 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ashraf Y. El-Dakar  1; Shymaa Mohamed Shalaby2; Neama Elaraby2
| ||||
| 1Faculty of Aquaculture and Marine Fisheries, Arish University. El-Arish, North Sinai, Egypt | ||||
| 2Faculty of Fish Resources, Suez University, Egypt | ||||
| Abstract | ||||
| Phosphorus is one of the most important nutritional factors which regulate cell growth and plays a significant role in most cellular processes. The effect of different phosphorus sources on the growth and biochemical composition of Nannochloropsis oculata (N. oculata) was investigated in this study. Four treatments: Mono potassium phosphate )KH₂PO4), potassium di phosphate (K2HPO4), calcium super phosphate (CaH6O9P2), and media without any phosphorus sources were all compared to F/2 media, which has sodium di hydrogen phosphate (NaH2PO4) as control at a concentration of 36 µmol -1. The experiment lasted for 15 days under the conditions of 100 μmol photons per m2s of light intensity, with a light-to-dark (L/D) ratio of24:0, a salinity 25 g/L, temperature maintained at 23± 1 °C, and a pH value of pH 7.5 ±0.3. The growth of N. oculata based on cell density was inhibited in the medium without any phosphorus sources (5.3×106 cell/ mL) and increased significantly in the medium with NaH2PO4 (16.4×106 cell/ mL). All phosphorus sources showed that there were significant differences (P ≤ 0.05) between all treatment media in chlorophyll content; KH₂PO4 achieved the highest significant chlorophyll a content (Chl a) with 4.25 mg/l, followed by K2HPO4 4.12 mg/l. Cells treated with NaH2PO4 produced the highest total protein and lipid content (31.14% and 46.63% by dry weight, respectively). Media without phosphorus had the highest total carbohydrate (22.48%), while the lowest total lipids and total carbohydrate achieved by KH₂PO4 and CaH6O9P2 had the lowest total protein (25.90%). | ||||
| Keywords | ||||
| N. oculata; Phosphorus Sources; Cell Density; Biochemical Composition; Fatty acids Profile | ||||
|
Statistics Article View: 84 PDF Download: 82 |
||||
