ACCOMPANIMENT OF THE POLYMORPHISM AT DEFENSING GENE LOCI WITH MILK TRAITS AND MILK SOMATIC CELL COUNT IN EGYPTIAN BUFFALO | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 79, Issue 4, December 2019, Page 913-920 PDF (245.88 K) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| The aim of this study is to investigate a polymorphism of the defensin gene and search for its association with daily milk traits and milk somatic cell count (SCC) in experimental buffalo population. Composite milk samples were collected monthly from fifty lactating buffaloes and analysed including fat, protein and lactose contents and somatic cell count. Samples were cultured for bacterial presence. The polymerase chain reaction-restriction fragment length polymerase (PCR-RFLP) technique utilizing Taq1 endonuclease enzyme was used on genomic DNA extracted from blood samples. Statistical analysis of dataset was performed using the mixed linear model. Within studied buffaloes, no significant associations were found between the RFLP-Taq1 and milk yield, fat and protein content and SCC.However, a significant effect was shown of the RFLP-Taq1 on the lactose content. | ||||
| Keywords | ||||
| Buffalo population; Defensin gene; Milk traits; Somatic cell count; PCR-RFLP | ||||
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