UPDATE ON BROILERS CHICKEN AND HUMAN ESBL VIRULENT SALMONELLA SEROVARS | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 79, Issue 4, December 2019, Page 973-995 PDF (593.52 K) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| The aim of this present study to survey the antimicrobial resistance, ESβL and virulence genes among Salmonella serovars isolated from broilers chickens and Human .300 broilers chickens and 60 stool human samples were investigated for Salmonella by cultural, biochemical and serotyping, 44 isolates were positive for Salmonella with the most predominant serotypes are S, Enteritidis and S, [Typhimurium in all isolates from broilers chickens and Human. Broilers Salmonella isolates showed high resistance to Impiniem (83.3%) followed by Ceftriaxone (73.3%),On contrast showed high sensitive to Cephalexin (73.3%).But in human isolates showed high resistance to Ampicillin-Sulbectam (21.4%) ,On contrast showed highsensitivetoCeftazedime,AmikacinandTrimethoprime-Sulphamethoxazole (100 %) for each. By using PCR test for detection of four virulence genes (invA,adrA,ompA and csgD)and nineresistancegenes (int1, int2, int3, BlaTEM, BlaCTX, BlaOXA, Mox,gyrA and gyrS), detected the presence of invA virulence and BlaTEM resistance gene in all serovars isolated from broilers chickens and human. Finally, in our study the results of genotypic and phenotypic analysis, found close relation between human and broilers chickens Salmonella strains | ||||
| Keywords | ||||
| Salmonella serovars; virulence gene; ESβL; broilers; human | ||||
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