Bacteriological and molecular studies on some bacterial agents from neonatal calf diarrhea
Abd El-Tawab, A., Hofy, F., Mohamed, S., Salim, R. (2017). Bacteriological and molecular studies on some bacterial agents from neonatal calf diarrhea. EKB Journal Management System, 33(2), 465-475. doi: 10.21608/bvmj.2017.30596
Ashraf A. Abd El-Tawab; Fatma I. Hofy; Sahar R. Mohamed; Reham A. Salim. "Bacteriological and molecular studies on some bacterial agents from neonatal calf diarrhea". EKB Journal Management System, 33, 2, 2017, 465-475. doi: 10.21608/bvmj.2017.30596
Abd El-Tawab, A., Hofy, F., Mohamed, S., Salim, R. (2017). 'Bacteriological and molecular studies on some bacterial agents from neonatal calf diarrhea', EKB Journal Management System, 33(2), pp. 465-475. doi: 10.21608/bvmj.2017.30596
Abd El-Tawab, A., Hofy, F., Mohamed, S., Salim, R. Bacteriological and molecular studies on some bacterial agents from neonatal calf diarrhea. EKB Journal Management System, 2017; 33(2): 465-475. doi: 10.21608/bvmj.2017.30596

Bacteriological and molecular studies on some bacterial agents from neonatal calf diarrhea

Benha Veterinary Medical Journal
Article 47, Volume 33, Issue 2, December 2017, Page 465-475  XML PDF (490.15 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2017.30596
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Authors
Ashraf A. Abd El-Tawab1; Fatma I. Hofy1; Sahar R. Mohamed2; Reham A. Salim3
1Bacteriology, Immunology and Mycology Dep., Fac. of Vet.Med., Benha Univ.
2Animal Health Research Institute, Doki, Giza
3Mashtoul –El souk veterinarian training center
Abstract
Neonatal Calf diarrhea(NCD) is a commonly reported disease and considered as a major cause of
economic loss to cattle producers. This study was done on 100 fecal swabs collected from
diarrheic calves(0 day-2monthes) old during the period from December 2015 till January 2017 and
subjected for bacteriological ,serological and molecular investigations. The infection rate of E.coli
was 47% followed by Pseudomonas aeruginosa( 4% )and Salmonella Typhimurum( 1%). Multi
drug resistant appeared in the most tested microorganisms. Serogrouping of E. coli revealed the
presence of O142 ,O55 ,O11,O27, O157,O119 ,O26 and O127 by a percentage of
20.69%,17.24%,13.8%,6.9%,6.9%.6.9%,3.45% and 3.45%, respectively. Multiplex PCR was
applied for detection of virulence genes stx1(5/10),stx2(3/10) and eae(6/10)) which detected in
E.coli and stn gene in Salmonella typhimurum and also (blaVIM( 3/4), mexR(4/4)) genes for
Pseudomonas aeruginosa were detected
Keywords
Calf diarrhea; E.coli; Pseudomonas aeruginosa; Salmonella typhimurum; Virulence &resistance genes
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