Paeonol Preconditioning Alleviates Partial Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Inhibition of Autophagy and TLR4/MyD88/NF-κB Pathway
Abdelsameea, A., Alsemeh, A., Desouky, M., Samy, W., Fawzy, A., Abbas, N. (2023). Paeonol Preconditioning Alleviates Partial Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Inhibition of Autophagy and TLR4/MyD88/NF-κB Pathway. EKB Journal Management System, 29(4), 983-993. doi: 10.21608/zumj.2022.173661.2684
Ahmed Ahmed Abdelsameea; Amira Ebrahim Alsemeh; Maha Desouky; Walaa Samy; Amal Fawzy; Noha A.T. Abbas. "Paeonol Preconditioning Alleviates Partial Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Inhibition of Autophagy and TLR4/MyD88/NF-κB Pathway". EKB Journal Management System, 29, 4, 2023, 983-993. doi: 10.21608/zumj.2022.173661.2684
Abdelsameea, A., Alsemeh, A., Desouky, M., Samy, W., Fawzy, A., Abbas, N. (2023). 'Paeonol Preconditioning Alleviates Partial Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Inhibition of Autophagy and TLR4/MyD88/NF-κB Pathway', EKB Journal Management System, 29(4), pp. 983-993. doi: 10.21608/zumj.2022.173661.2684
Abdelsameea, A., Alsemeh, A., Desouky, M., Samy, W., Fawzy, A., Abbas, N. Paeonol Preconditioning Alleviates Partial Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Inhibition of Autophagy and TLR4/MyD88/NF-κB Pathway. EKB Journal Management System, 2023; 29(4): 983-993. doi: 10.21608/zumj.2022.173661.2684

Paeonol Preconditioning Alleviates Partial Hepatic Ischemia/Reperfusion Injury in Rats: The Role of Inhibition of Autophagy and TLR4/MyD88/NF-κB Pathway

Zagazig University Medical Journal
Article 2, Volume 29, Issue 4, July and August 2023, Page 983-993  XML PDF (1.24 MB)
Document Type: Original Article
DOI: 10.21608/zumj.2022.173661.2684
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Authors
Ahmed Ahmed Abdelsameea email orcid 1; Amira Ebrahim Alsemehorcid 2; Maha Desouky3; Walaa Samy4; Amal Fawzy4; Noha A.T. Abbas5
1Assistant professor of clinical pharmacology, clinical pharmacology Department, Zagazig University, Zagazig, Egypt, E mail :
2Anatomy and embryology,faculty of medicine,zagazig university
3Department. of Anatomy , Faculty of Medicine, Minia University, Egypt
4Medical Biochemistry Department, Faculty of Medicine-Zagazig University, Zagazig, Egypt.
5Department of Clinical Pharmacology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
Abstract
Background: Liver injury due to ischemia/reperfusion (I/R) is a major challenge during liver surgery. Objectives: Effects of paeonol pretreatment on partial hepatic I/R injury were assessed in this study. Methods: Male Wister rats were allocated into sham, vehicle-I/R, and paeonol-I/R groups. Paeonol, 100 mg/kg/day, was administered by gavage. After 10 days, rats of I/R groups were subjected to partial I/R of the liver. Blood samples were collected for assessment of alanine and aspartate aminotransferases as well as gamma glutamyl transferases. Liver samples were taken for detection of tumor necrosis factor-α (TNF-α) and malondialdhyde (MDA) levels as well as superoxide dismutase (SOD) activity. The mRNA expression of toll-like receptor-4 (TLR4), myeloid differentiation factor 88 (MyD88), high mobility group box 1 (HMGB1), and peroxisome proliferator-activated receptor-γ (PPAR-γ) were assessed in the liver. Hematoxylin& eosin stained liver sections were examined. Expressions of nuclear factor kappa B (NF-κB) and autophagy markers (LC3 II, P62) were assessed in immunostained sections. Results: Liver I/R increased serum transferases and tissue MDA, TNF-α, TLR4, HMGB-1, MyD88, NF-κB, LC3 II, P62 levels with hydropic degeneration and cellular infiltration in the liver while decreased SOD activity and PPAR-γ mRNA expression. These alterations were attenuated by paeonol pretreatment. Conclusions: Paeonol pretreatment alleviated liver I/R injury through inhibition of autophagy and TLR4/MyD88/NF-κB pathways.
Keywords
Reperfusion; Autophagy; Oxidative stress; Paeonol; Ischemia
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