Prevalence of Bacterial Infections in Neonatal Intensive Care Unit and Genetic Characterization of Their Drug Resistance | ||||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||||
| Article 3, Volume 28, Issue 1, June 2023, Page 33-44 PDF (1.12 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/scvmj.2023.307329 | ||||
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| Authors | ||||
Mohamed Abdel-Razik1; Eslam Aboulhamd Hafez  2; Mohamed Ahmad Hassan3
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| 1Department of Botany and Microbiology, Faculty of Science, Suez Canal University, Egypt. | ||||
| 2Department of Botany and Microbiology, Faculty of Science, Suez Canal University, Egypt. 3- Department of Pediatrics and Neonatology, Faculty of Medicine, Sohag University, Egypt | ||||
| 3Department of Pediatrics and Neonatology, Faculty of medicine, Sohag University, Egypt | ||||
| Abstract | ||||
| Out of the 120 blood samples collected from investigated neonates, 40 samples were positive for bacteria. Blood culture was carried out using BACT/ ALERT (3D 60) then the positive cultures were subculture on MacConkey, Blood, and Sabouraud dextrose agar. Biochemical tests were used to identify the isolates, and the automated Vitek 2 system provided confirmation. The incidence of neonatal sepsis was predominantly due to gram-negative bacteria (75%) mainly K. pneumoniae (60 %), followed that E. coli (7.5 %). While all gram-positive bacteria (25 %) are represented by CONS. As stated by the Clinical and Laboratory Standards Institute (CLSI) guidance, an antimicrobial susceptibility test using the disc diffusion method (Kirby-Bauer) was conducted to select multi-drug resistant bacteria as (ESβL) producers. The ESβL genes responsible for the resistance were detected using the PCR method. It was determined that among ESβL of K. pneumoniae and E. coli, the blaTEM and blaCTX-M genes were the predominant gene (100 %), followed by blaSHV (80 %). | ||||
| Keywords | ||||
| NICU; ESBL; Sepsis; K. pneumoniae | ||||
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