Molecular Detection of Human Norovirus among Children (0-5 Years) Attending Selected Hospitals in Kano Metropolis, Kano State, Nigeria

Kabir Abubakar, Nusaiba K.; Abdelrahman, Taysir R.; Idris Kabuga, Auwal I.; Dahiru Rogo, Lawal D.

doi:10.21608/ejmm.2023.307443

	Molecular Detection of Human Norovirus among Children (0-5 Years) Attending Selected Hospitals in Kano Metropolis, Kano State, Nigeria
Egyptian Journal of Medical Microbiology
Volume 32, Issue 3, July 2023, Page 87-97 PDF (445.43 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2023.307443
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Authors
Nusaiba K. Kabir Abubakar¹; Taysir R. Abdelrahman ²; Auwal I. Idris Kabuga¹; Lawal D. Dahiru Rogo³
¹Department of Medical Microbiology and Parasitology, Faculty of Clinical Sciences, Bayero University, Kano Nigeria
²Department of Medical Microbiology and Parasitology, Faculty of Clinical Sciences, Bayero University, Kano Nigeria; Deoartment of Medical Microbiology and immunology, Faculty of Medicine, Al-Azhar University, Cairo Egypt
³Department of Medical Microbiology and Parasitology, Faculty of Allied Sciences, Bayero University, Kano Nigeria
Abstract
*Background:* Norovirus has been reported as the main cause of acute diarrhea worldwide after rotavirus. But its incidence in developing countries such as Nigeria has been underreported. Objectives: This study aimed to detect human Norovirus genogroups I, II and IV in stools of children (0-5 years) with diarrhea in three selected Hospitals (Muhammad Abdullahi Wase Specialist Hospital, Murtala Muhammad Specialist Hospital, and Hasiya Bayero Pediatric Hospital) in Kano, Nigeria, estimate the prevalence of Norovirus infection, determine the socio-demographic characteristics and other factors and examine the clinical profile of the study children in relation to their infection statuses. Methods: Two hundred (200) diarrheic fecal samples were collected from 0-5 years children between June-October 2021. The samples were examined for NoV using one-step real time reverse transcription-Polymerase chain reactions (RT-PCR) technique. Results: In this study, the total prevalence of Norovirus infection was found to be 21%, with Genogroups II having the highest frequency of 25 (12.5%), followed by Genogroup I having 11(5.5%), and Genogroup IV with the lowest 6(3.0%). Our investigation identified co-infections of three Norovirus genogroups: I and II (0.5%), I and IV (0.5%), and I, II and IV 2(1.0%). Conclusion: According to this study, Norovirus Genogroup II is the most common strain circulating in Kano, Nigeria, and it has been established that NoV is a relevant cause of pediatric diarrhea. However, there isn't any evidence linking the Norovirus to any other risk factors. In order to stop the spread of NoV, it is essential to keep the environment clean and further research is needed with more samples to find if possible link between risk factors and the presence of the virus.
Keywords
Norovirus; Genogroups; Children; Kano metropolis; One-Step Real-time RT-PCR; Diarrhea; Acute gastroenteritis


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