Molecular detection and phylogenetic analysis of recent isolate of lumpy skin disease virus from clinical cases in Menofia governorate between 2018 to 2020 based on GPCR gene.

Abdelsalam, Adel Elsayed; El-Habbaa, Ayman Said; Abo Hatab, Eman Mohamed; Sharawy, Saad Sharawy Ali

doi:10.21608/bvmj.2023.206872.1652

	Molecular detection and phylogenetic analysis of recent isolate of lumpy skin disease virus from clinical cases in Menofia governorate between 2018 to 2020 based on GPCR gene.
Benha Veterinary Medical Journal
Volume 44, Issue 2, July 2023, Page 84-87 PDF (1.04 MB)
Document Type: Original Article
DOI: 10.21608/bvmj.2023.206872.1652
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Authors
Adel Elsayed Abdelsalam ¹; Ayman Said El-Habbaa ²; Eman Mohamed Abo Hatab ³; Saad Sharawy Ali Sharawy ⁴
¹1Department of Virology, Faculty of Veterinary Medicine, Benha University, Moshtoher, Benha, Egypt 2Virology Department, Animal Health Research Institute, Agricultural Research Center, Giza, Egypt
²Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohour, Kalubeya
³Virology Department, Animal Health Research Institute, Agricultural Research Center, Giza, Egypt
⁴Department of Virology, Faculty of Veterinary Medicine, Benha University, Moshtoher, Benha, Egypt
Abstract
Lumpy skin disease (LSD) is a viral illness of cattle population that causes highly significant losses in economy. In recent times, the illness has spread throughout various countries of Middle East, Egypt is among of them. The purpose of present research was to investigate the genetic make-up of LSD virus during outbreaks of Menofia governorate from period between 2018 to 2020 and its association to wild field and vaccination strains and isolates in database. Nodular skin tissues (n = 30), whole blood (n=50) and milk (n=10) samples were taken from apparently diseased cows for LSDV molecular detection based on GPCR gene. By PCR testing, 60 of the 90 samples tested were positive for LSDV. The amplified products of 7 positive samples found in the nodular tissue (n= 3 ), buffy coat (n= 3) and milk (n= 1) of cattle from Menofia governorate were subjected to sequencing and thus the evolutionary tree was built. Furthermore, the sequence alignment of the Menofia governorate LSDV strains revealed remarkable similarity ranging from 99% to 100% in between and with the LSDV genome of database strains and 95% to 96 % with vaccinal strains. To summarise GPCR gene sequencing and phylogenetic investigations showed only minor genetic differences among LSDV wild strains from various areas, as well as a close link between virulent field strains and homologous vaccines.
Keywords
LSDV; PCR; Phylogenetic analysis; Egypt


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