Isolation and Identification of Lumpy Skin Disease Virus from Cattle in Menofeia Governorate during May 2019 to January 2020.
Rashed, E., Shemies, O., Ali Sahrawi, S. (2023). Isolation and Identification of Lumpy Skin Disease Virus from Cattle in Menofeia Governorate during May 2019 to January 2020.. EKB Journal Management System, 44(2), 93-96. doi: 10.21608/bvmj.2023.187231.1628
Eman El Sayed Rashed; Omaima Abdel Azeez Shemies; Saad Sharawi Ali Sahrawi. "Isolation and Identification of Lumpy Skin Disease Virus from Cattle in Menofeia Governorate during May 2019 to January 2020.". EKB Journal Management System, 44, 2, 2023, 93-96. doi: 10.21608/bvmj.2023.187231.1628
Rashed, E., Shemies, O., Ali Sahrawi, S. (2023). 'Isolation and Identification of Lumpy Skin Disease Virus from Cattle in Menofeia Governorate during May 2019 to January 2020.', EKB Journal Management System, 44(2), pp. 93-96. doi: 10.21608/bvmj.2023.187231.1628
Rashed, E., Shemies, O., Ali Sahrawi, S. Isolation and Identification of Lumpy Skin Disease Virus from Cattle in Menofeia Governorate during May 2019 to January 2020.. EKB Journal Management System, 2023; 44(2): 93-96. doi: 10.21608/bvmj.2023.187231.1628

Isolation and Identification of Lumpy Skin Disease Virus from Cattle in Menofeia Governorate during May 2019 to January 2020.

Benha Veterinary Medical Journal
Volume 44, Issue 2, July 2023, Page 93-96  XML PDF (1.13 MB)
Document Type: Original Article
DOI: 10.21608/bvmj.2023.187231.1628
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Authors
Eman El Sayed Rashed email 1; Omaima Abdel Azeez Shemies2; Saad Sharawi Ali Sahrawiorcid 3
1Department of Virology, Faculty of Veterinary Medicine, Banha University, Moshtoher, Banha, Egypt
2Department of Virology, Animal Health Research Institute, Dokki, Egypt.
3Department of Virology, Faculty of Veterinary Medicine, Banha University, Banha, Qualubia, Egypt
Abstract
ABSTRACT
Lumpy skin disease virus (LSDV) is a significant and serious viral skin disease affecting cattle. In this study, isolation and identification of LSDV from ten skin nodule samples collected from infected cattle at different localities of Menofeia province, Egypt between May 2019 and January 2020. Seven from ten nodular samples gave characteristic pock lesions by isolation of LSDV on chorioallantoic membrane (CAM) of specific pathogen free embryonated chicken eggs (SPF-ECEs),while four from ten nodular samples gave prominent CPE at the third passage on Madin Darby Bovine Kidney) MDBK (cell culture. Seven isolated samples tested positive by Indirect Fluorescent Antibody test (IFAT). Using specific primers for the LSDV envelope protein (P32) gene, polymerase chain reaction (PCR) detected LSDV nucleic acid in seven of ten isolated samples and confirmed an LSDV isolate with a specific amplified product of 192 bp. Accurate and fast diagnosis of LSD is critical for controlling the rapid spread of the disease. PCR is effective, quick, and precise method for detecting DNA of LSDV in clinical specimens, such as skin nodules, CAM and MDBK cells. Mass vaccination should be applied for cattle in Egypt using effective vaccines like the attenuated Neethling strain vaccine.
Keywords
Keywords: LSDV; Cattle; CAM; MDBK; PCR
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