Assessment of the accuracy of identification of clinical Candida species with reduced fluconazole susceptibility by rapid methods and their relative expression of major efflux pump genes
Aboulela, A., Idris, S., Metwally, D., Emad, R., El Sawaf, G. (2023). Assessment of the accuracy of identification of clinical Candida species with reduced fluconazole susceptibility by rapid methods and their relative expression of major efflux pump genes. EKB Journal Management System, 4(3), 994-1007. doi: 10.21608/mid.2023.220044.1553
Aliaa Gamaleldin Aboulela; Samah Nasr Idris; Dalia El Sayed Metwally; Rasha Emad; Gamal El Din Ahmed El Sawaf. "Assessment of the accuracy of identification of clinical Candida species with reduced fluconazole susceptibility by rapid methods and their relative expression of major efflux pump genes". EKB Journal Management System, 4, 3, 2023, 994-1007. doi: 10.21608/mid.2023.220044.1553
Aboulela, A., Idris, S., Metwally, D., Emad, R., El Sawaf, G. (2023). 'Assessment of the accuracy of identification of clinical Candida species with reduced fluconazole susceptibility by rapid methods and their relative expression of major efflux pump genes', EKB Journal Management System, 4(3), pp. 994-1007. doi: 10.21608/mid.2023.220044.1553
Aboulela, A., Idris, S., Metwally, D., Emad, R., El Sawaf, G. Assessment of the accuracy of identification of clinical Candida species with reduced fluconazole susceptibility by rapid methods and their relative expression of major efflux pump genes. EKB Journal Management System, 2023; 4(3): 994-1007. doi: 10.21608/mid.2023.220044.1553

Assessment of the accuracy of identification of clinical Candida species with reduced fluconazole susceptibility by rapid methods and their relative expression of major efflux pump genes

Microbes and Infectious Diseases
Article 34, Volume 4, Issue 3, August 2023, Page 994-1007  XML PDF (918.77 K)
Document Type: Original Article
DOI: 10.21608/mid.2023.220044.1553
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Authors
Aliaa Gamaleldin Aboulela email orcid 1; Samah Nasr Idris1; Dalia El Sayed Metwallyorcid 2; Rasha Emadorcid 3; Gamal El Din Ahmed El Sawaf1
1Microbiology Department, Medical Research Institute, Alexandria University, Egypt
2Microbiology Department, Medical Research Institute, Alexandria University
3Alexandria Main University Hospital, Alexandria, Egypt
Abstract
Background: Candida infections are commonly treated with fluconazole. Efflux pumps are one of the mechanisms for fluconazole resistance. This study assessed the accuracy of identification of Candida species with reduced fluconazole susceptibility and their relative expression of major efflux pump genes. Methods: Candida species (n=111) were collected from clinical samples, identified by Brilliance Candida Agar (BCA) and germ tube test, then tested for fluconazole susceptibility by disk diffusion. Confirmation of identification and susceptibility to fluconazole was done by Phoenix BD and broth microdilution methods, and the accuracy of preliminary methods was calculated. qPCR was performed to evaluate the gene expression of common efflux pump genes. Results: The most predominant were C. albicans (32%) and C. tropicalis (29%). Accuracy of BCA identification was 84% for both species. Reduced fluconazole susceptibility was detected in 8.3% of C. albicans and 40.6% of C. tropicalis. Accuracy of disk diffusion was 100% for C. albicans and 62% for C. tropicalis. qPCR showed upregulation of CDR2 efflux pump among non-susceptible C. albicans (1.99 ± 1.04), though not statistically significant. CDR1 expression was at a basal level for C. albicans (0.9 ± 0.25) and C. tropicalis (0.69 ± 0.76). MDR-1 was significantly downregulated in non-susceptible C. tropicalis (0.30 ± 0.63, p= 0.025). Conclusion: Brilliance Candida Agar  identification requires verification. The disk diffusion method is of higher accuracy with C. albicans than with C. tropicalis. Overexpression of CDR1 and CDR2 in C. albicans, and CDR1 and MDR1 in C. tropicalis were not the main reasons behind reduced susceptibility to fluconazole.
Keywords
C. albicans; C. tropicalis; CDR1,CDR2 and MDR1; Fluconazole resistance
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