Comparative Effect of Stevia and Aspartame Sweeteners: A Biochemical, Sero-Biochemical, Histochemical and Immunohistochemical Study | ||||
| Suez Canal Veterinary Medical Journal. SCVMJ | ||||
| Article 9, Volume 28, Issue 1, June 2023, Page 115-131 PDF (1.12 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/scvmj.2023.307764 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Eman Sameh  1; kawther Elhady 2; Sahar M. Srour3; Reham El-Tarabili4; Ahmed Shaker Eltahan5; Rania H. Abdou2
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| 1Free Veterinarian | ||||
| 22Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine,Suez Canal University, Ismailia 41522, Egypt | ||||
| 3Biochemistry and Toxicology Department, Animal Health Research, Institute | ||||
| 4Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt | ||||
| 5Department of Pathology, Faculty of Veterinary Medicine,Suez Canal University, Ismailia 41522, Egypt | ||||
| Abstract | ||||
| Aspartame is a common artificial sweetener that is thought to be dangerous. On the other hand, stevioside (stevia) a natural sweetener of plant origin was assumed to be a natural alternative to artificial sweeteners. This study aimed to determine whether aspartame or stevioside affected biochemical, serum-biochemical, and immunohistochemical changes in adult rats following six weeks of exposure. Forty-eight rats were used and divided into three groups: Group I: the control group, Group II: rats received aspartame (75 mg/kg/day), Group III: rats received stevioside (45 mg/kg/day). All medications were given orally for 6 weeks. Lipid Peroxidation product (MDA); liver glutathione (GSH); nitric oxide (NO); Liver Superoxidase dismutase activity(SOD); catalase (CAT), Low-density lipoprotein (LDL); very low lipoprotein (VLDL); the cholesterol in the HDL; Alanine Aminotransferase (ALT), and Aspartate Aminotransferase (AST); Creatinine, Urea, Total proteins were assessed in blood and liver tissue specimens. In addition, hepatic histopathological and immunohistochemical investigations were done. The Aspartame group showed a significant increase in most of the investigated biochemical parameters (especially glucose, MDA, liver NO, LDL, VLDL, ALT, AST, creatinine, and urea) with a significant decrease in serum albumin and the endogenous antioxidants (GSH, SOD, CAT) compared to the control group while stevia-exposed animals showed a significant decrease in the same biochemical parameters with a significant increase in the same endogenous antioxidants. Moreover, severe histopathological and immunohistochemical changes were observed in the liver of Aspartame- treated animals. However, Stevia-treated rats showed the least changes. Therefore, it can be concluded that Stevia was less harmful than aspartame. | ||||
| Keywords | ||||
| Aspartame; Stevia; Sweeteners; Immunohistochemical; Biochemical | ||||
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