Comparison of Cell Dynamics and Characterization of Bone Marrow-Derived Stem Cells in Different Passages in Young and Aged Rats | ||||
| Suez Canal University Medical Journal | ||||
| Article 3, Volume 26, Issue 5, May 2023, Page 18-33 PDF (1.32 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/scumj.2023.308017 | ||||
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| Authors | ||||
| Rania A. Galhom1, 2; Mona F. Mansour2, 3; Noha M. Abd-El Fadeal2, 4; Angie M. Ameen2, 3; Noura R. Abd El-Hamid2, 5; Nahla Imbarak2, 6; Eman A. Mohammed2, 5; Sally S. Mohammed* 2, 6 | ||||
| 1Human Anatomy and Embryology Department, Faculty of Medicine, Suez Canal University, Egypt | ||||
| 2Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, Egypt | ||||
| 3Human Physiology Department, Faculty of Medicine, Suez Canal University, Egypt | ||||
| 4 Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Suez Canal University, Egypt | ||||
| 5Medical Genetics Unit, Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University, Egypt | ||||
| 6Histology and Cell Biology Department, Faculty of Medicine, Suez Canal University, Egypt | ||||
| Abstract | ||||
| Background: Bone marrow-derived mesenchymal cells (BM-MSCs) isolation and culturing have a great interest in many diseases as cell therapy. The clinical use of cultured stem cells is facing debates and criticism due to the increasing number of unproven cell-based therapies and the appearance of many limitations, including the age of the donor and the number of passages. Aim: Due to the discrepancy in the published data, we investigated changes associated with the first four passages of cultured BM-MSCs from old and young rats, regarding morphological and ultrastructure changes, growth kinetics, and surface cell markers. Methods: Cells were isolated and cultured from young and old Wistar rats’ long bones. The morphological and ultrastructural characters, cell surface markers, and growth kinetics of the stem cells were analyzed. Results: BM-MSCs in P1 and 2 showed non-significant differences between young and old cells. Morphologically, cells from P3 and P4 became larger and filled with filaments in both groups. The capacity of the cells to proliferate decreased compared to the prior passages, though, it is still better in the young population. Immunophenotypically, young source of cells showed a significant expression of MSCs surface markers across different passages reaching at least 50% of the cells by P4. While steady expression was experienced in old cells in different passages. Conclusion: the donor age directly influences BM-MSCs morphology, ultrastructure, and proliferation. We assert that cells obtained from P2 and P3 of young rats have the best proliferative index which can be used for future studies. | ||||
| Keywords | ||||
| Cell dynamics; Electron microscope; Flow Cytometry; Mesenchymal stem cells; Phenotyping | ||||
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