Molecular diagnosis of Type I diabetes in some Egyptian patients using HLA-DRB1 exon 2 gene
Z.Higazy, K., M.Abdel-kareim, A., F.Al-Husseini, N., H.Awwad, M. (2022). Molecular diagnosis of Type I diabetes in some Egyptian patients using HLA-DRB1 exon 2 gene. EKB Journal Management System, 7(12), 63-75. doi: 10.21608/bjas.2022.308845
Kholud Z.Higazy; Amal M.Abdel-kareim; Naglaa F.Al-Husseini; Mohammed H.Awwad. "Molecular diagnosis of Type I diabetes in some Egyptian patients using HLA-DRB1 exon 2 gene". EKB Journal Management System, 7, 12, 2022, 63-75. doi: 10.21608/bjas.2022.308845
Z.Higazy, K., M.Abdel-kareim, A., F.Al-Husseini, N., H.Awwad, M. (2022). 'Molecular diagnosis of Type I diabetes in some Egyptian patients using HLA-DRB1 exon 2 gene', EKB Journal Management System, 7(12), pp. 63-75. doi: 10.21608/bjas.2022.308845
Z.Higazy, K., M.Abdel-kareim, A., F.Al-Husseini, N., H.Awwad, M. Molecular diagnosis of Type I diabetes in some Egyptian patients using HLA-DRB1 exon 2 gene. EKB Journal Management System, 2022; 7(12): 63-75. doi: 10.21608/bjas.2022.308845

Molecular diagnosis of Type I diabetes in some Egyptian patients using HLA-DRB1 exon 2 gene

Benha Journal of Applied Sciences
Article 8, Volume 7, Issue 12, December 2022, Page 63-75  XML PDF (696.58 K)
Document Type: Original Research Papers
DOI: 10.21608/bjas.2022.308845
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Authors
Kholud Z.Higazy1; Amal M.Abdel-kareim1; Naglaa F.Al-Husseini2; Mohammed H.Awwad1
1Zoology Dept., Faculty of Science, Benha University, Egypt
2Biochemistry Dept., Faculty of medicine, Benha University, Egypt
Abstract
             Type 1 diabetes is regarded as a chronic autoimmune disease characterized by dysfunctional T-lymphocyte activation that targets pancreatic Langerhans β-cells, causing insulin deficiency. By raising the amounts of sugar in the blood and urine, this condition causes hyperglycemia. Five groups were chosen for the current experiment, with N standing in for healthy individuals. Four groups of patients were created based on their ages. The bidirectional sequence of the HLA-DRB1 exon 2 gene was determined using blood samples from the five groups. PCR was used to define the gene sequence, amplify it, and isolate the gene for DNA sequencing in two directions. To understand more about the isolated gene and its role in T1DM, bioinformatics analysis of the sequencing output data was carried out. In addition, certain software programs were used by the study teams to forecast the translated protein and RNA secondary structure. The findings showed that an alteration in the secondary structure of RNA affected protein translation and release in bodily cells. Additionally, based on SNP analysis, the four groups' targeted genes revealed mutations caused by the replacement or indel of certain nucleotides, which change the anticipated translated protein in patient groups in comparison to control.
Keywords
Type 1 diabetes; HLA-DRB1; exon 2; DNA sequencing; autoimmune disease
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