NOVEL MEDIA TO IDENTIFY SORBITOL NEGATIVE E. COLI TO EASE DETECTION E. COLI O157:H7. | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 77, Issue 3, September 2017, Page 435-442 PDF (379.08 K) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| Identification of Escherichia coli is an important task in both public health and Clinical microbiology laboratories. E.coli is responsible for a wide variety of diseases in human and animals, including diarrhea, septicemia, hemorrhagic enteritis, respiratory diseases and ear infections. Pathogenic isolates of E.coli are of special significance, therefore, a rapid, inexpensive method to presumptively identify E.coli isolates with a high specificity and sensitivity is desirable. We developed a single-tube method as a screening test for E.coli sorbitol-negative from various clinical human stool, bovine feces and food specimens also could be used for environmental samples. In the present study novel: cellobiose-inositol-sorbitol- Iron-indole agar used as a new medium (in single tube) as a selective/differntional to identify sorbitol negative E. coli to ease detection E. coli O157:H7 versus conventional identification. The aim of study: To evaluate a novel medium in a single tube, for screening isolates suspected to possibly represent sorbitol negative E.coli and comparison of its results to results of the classical biochemical reactions (FDA) procedure includes: TSI, LIA, MIO, Urea, Simmons Citrate and sorbitol tube. The novel medium that screens sorbitol negative E. coli in single tube will be cost effective and time saving procedure to ease detection of serotype O157 that is why we selected this new medium as a subject of study. | ||||
| Keywords | ||||
| E.coli, Screening; Gram-negative bacilli; Culture media | ||||
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