IDENTIFICATION OF ANTIBIOTIC RESISTANCE GENES FOR CAMPYLOBACTER FETUS ISOLATED FROM INFERTILE AND ABORTED BOVINES IN SOME EGYPTIAN GOVERNORATES | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 77, Issue 3, September 2017, Page 467-481 PDF (1.73 MB) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| In this study, 266 samples including 81 preputial swabs, 34 semen samples from bulls and 118 vaginal swabs, 16 uterine discharges and 17 aborted foeti from dairy cows suffering from infertility and abortion were randomly collected from January - December 2016 from some governorates in Egypt.The antimicrobial susceptibilities were applied against 12 antimicrobial agents.All the Campylobacter isolates were tested for the presence of AphA -3-1and blaOXA- 61 genes. The results showed that Campylobacter isolates were 52(19.55%) of the total samples (n= 266) with prevalence of Campylobacter fetus subspecies venerealis (CFV), 32 (61.54%),followed byCampylobacter fetus subspecies fetus (CFF),20 (38.64%). The highest incidence of Campylobacter spp. was (37.5%) in uterine discharges, followed by aborted foeti (23.53%) then in vaginal swabs (22.88%) and in preputial swabs (18.52%) respectively. The highest incidence of CFV was in uterine discharges (25%), aborted foeti (17.65%), and vaginal swabs (13.56%) and in preputial swabs (11.11%) respectively. Out of the Campylobacter isolates, 23 (44.23%) showed the right band (701 bp) that which denotes phenotypic resistance to erythromycin is contained AphA gene. Twelve (23.07%) out of 52 Campylobacter isolates showed the right band (372 bp) which is contained the blaOXA- 61 gene that denotes phenotypic resistance to ampicillin. Finally, we have concluded that the existence of antimicrobial resistant genes in Campylobacter spp. will reduce the efficacy of several classes of antimicrobials commonly used in the treatment resulting in high morbidity and mortality rates associated with subsequent economic losses due to the use of ineffective antimicrobials. | ||||
| Keywords | ||||
| Campylobacter fetus subspecies venerealis; Campylobacter fetus subspecies fetus; antimicrobial resistance genes | ||||
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