MOLECULAR DETECTION OF BABESIOSIS AND ITS OXIDATIVE STRESS IN BLOOD OF INFECTED CATTLE | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 77, Issue 3, September 2017, Page 583-605 PDF (1.14 MB) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| This study was conducted on a total of 210 cattle. Eighty cattle were suspected for Babesia infection collected from private farms in Giza governorates and 130 from apparently healthy cattle before slaughtering at El Bassatine abattoir Cairo, Egypt. Microscopic examinations revealed that out of 210 cattle, 34 (16.19%) were found to be infected with Babesia species. Serological examinations (ELISA) revealed 54 (25.71%) were positive for babesia species antibodies. Ten blood samples (5 positive and 5 negative) were conducted for PCR amplification. All samples, that were positive by microscopical and serological examinations were also positive by PCR after using Babesia species specific primer (Babesia common) and specific primer for Babesia bovis at 644pb and 711bp respectively. Whereas, 3 out of 5 negative samples were positive by PCR by using Babesia species specific primer (Babesia common) and only 2 samples were positive by using specific primer for Babesia bovis. After parasitological and serological examinations, 20 samples highly positive for Babesia was subjected to hematological and biochemical analysis. In addition, samples from 10 microscopically negative healthy cattle were subjected to the same examination as control. Hematological examination showed a significant (P<0.05) decrease in red blood corpuscles count, hemoglobin, packed cell volume accompanied by a significant increase in total leucocytic count, neutrophils, eosinophils, monocytes and lymphocytes numbers. Blood analysis of positive samples for babesiosis revealed a significant (P<0.05) increase in malondialdehyde and nitric oxide levels accompanied by a significant (P<0.05) decrease of glutathione, superoxide dismutase, glucose-6- phosphate dehydrogenase as well as iron, cupper and vitamin E. Biochemical analysis of positive cases revealed that significant increase in, globulin, aminotranseferases alkaline phosphatase and lactate dehydrogenase activities, T.bilirubin, cholesterol, triglyceride, urea, creatinine whereas a significant(P<0.05) decrease in albumin, A/G ratio and glucose were recorded in comparison to the control group. | ||||
| Keywords | ||||
| Babesia; cattle; blood film; ELISA; PCR; oxidative stress; hematology; biochemistry | ||||
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