MICROBIOLOGICAL STUDIES ON MYCOPLASMA ISOLATED FROM EPIDIDYMIS OF CAMELS (CAMELUS DROMEDARIES) | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 77, Issue 4, December 2017, Page 785-795 PDF (1.21 MB) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| In the present study, 48 testicles and sera of apparently healthy male camels were collected separately from El-Basateen abattoir, Cairo, Egypt. The testicles of different camels were aseptically separated and examined microbiologically for the presence of Mycoplasma. Mycoplasma species was isolated and identified biochemically from 10.41% (5/48) of the total collected samples. Further confirmation of the isolates with molecular characterization using PCR revealed 8.3% (4/48) of the collected samples were confirmed as Mycoplasma bovis. Serological investigation using indirect ELISA against Mycoplasma bovis antigen revealed a prevalence rate of 41.66% (20/48). ELISA found to be a rapid and applicable technique while PCR was confirmatory and more specific for the detection of Mycoplasma bovis infection in male camel genitalia.The minimum inhibitory concentration of antibacterial agents revealed that Mycoplasma bovis isolates were most sensitive to Enrofloxacin and Tylosin. The study focused the attention on the impact of Mycoplasma bovis infection as one of the suspected causes of infertility and or reproductive disorders in male dromedary camels. | ||||
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