MOLECULAR EPIDEMIOLOGY OF SALMONELLA IN EGYPTIAN POULTRY FARMS | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 77, Issue 4, December 2017, Page 821-838 PDF (570.85 K) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| This study was designated to investigate the distribution of Salmonella in different chicken farms broiler, layer and breeder of different ages through bacteriological examination for different types of samples and to detect zoonotic serotypes of Salmonella by Polymerase Chain Reaction assay. A total number of 263 samples. (171 organ samples as fallow:82 livers, 51 yolk sac, 20 spleens, 16 ovaries, 2heart and 90 fecal swap and 2 litter samples) were obtained from 93 different poultry farms in different localities at6 governorates Qalubia, Sharkya, Minofia, Gharbya, Esmailia, Dakhlia and Giza during the period from 2013 to 2015. The samples were collected under complete aseptic condition from chickens suspected to be infected with salmonellosis. The incidence of Salmonella among chicken farms was 5.3% (14/263). It was (9.4%) among the broiler farms and (2.9%) among the layer farms by conventional culture methods. The results obtained showed that, the incidence of Salmonella in different organ samples were as follows: 7.47% among liver samples and it considered the highest incidence of Salmonella isolation followed by yolk sac 2% while the lowest rate of Salmonella isolation was from the spleen 0.21%, no isolation from heart, ovary, fecal swabs and litter samples and it is belonging to four serotypes. S. Enteritidis and S. Typhimurium indicated the highest incidence (42.85% and 28.57% respectively), will the other serovars S. Kentuky and S. Muenster were lower in incidence (21.42% and 7.14% respectively). The detection of (invA) gene provides that, all isolates were positive for it except two isolate. | ||||
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