CHARACTERIZATION AND PATHOGENICITY OF VIBRIO ALGINOLYTICUS ISOLATED FROM DISEASED CULTURED GILTHEAD SEABREAM | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 77, Issue 4, December 2017, Page 865-877 PDF (714.19 K) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| Fifty farmed gilthead seabream and two water samples were collected from two semi-intensive marine farms at Damietta province, Egypt during 2016. Fourteen Vibrio alginolyticus isolates were recovered and presumptively identified using morpho - chemical characterization then definitely confirmed by PCR targeting species specific collagenase gene.The total prevalence of the examined fish was 28% (14/50). All strains were resistant to ampicillin, oxacillin, tobramycinand kanamycin. On the other hand, all strains were sensitive to levofloxacin, gatifloxacin, doxycycline, oxolinic acid, flumequine and trimethoprim/sulfamethoxazole. The LD50 of Vibrio alginolyticus isolate (VAS7) were 3 × 105 CFUg-1 of fish body weight. | ||||
| Keywords | ||||
| Seabream; Vibrio alginolyticus; collagenase gene; Antibiogram; LD50 | ||||
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