CHARACTERIZATION OF VIBRIO PARAHEMOLYTICUS RETRIEVED FROM SEABREAM (SPARUS AURATA L.) DURING CLINICAL OUTBREAKS AT A SEMI-INTENSIVE FISH FARMS | ||||
Journal of the Egyptian Veterinary Medical Association | ||||
Volume 77, Issue 4, December 2017, Page 879-889 PDF (777.96 K) | ||||
Document Type: Original Research Articles | ||||
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Abstract | ||||
During 2016, a semi-intensive marine fish farms at Damietta province facing unknown clinical outbreaks. Vibrio parahemolyticus was the predominant bacterial isolates recovered from the internal organs of moribund seabream (Sparus aurata). Eight Vibrio parahemolyticus isolates were identified using phenotypic criteria, biochemical characterization and confirmed by PCR targeting species specific toxR genes. All strains were resistant to ampicillin, oxacillin, tobramycin and kanamycin and were suscetible to levofloxacin, gatifloxacin, doxycycline, oxolinic acid, flumequine and trimethoprim/sulfamethoxazole based on in-vitro antibiogram. The LD50 of Vibrio parahemolyticus isolate (VP3) was 1.0 × 106 CFU/g of fish body weight. | ||||
Keywords | ||||
Sparus aurata; Vibrio parahemolyticus; ToxR gene; LD50 | ||||
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