New antimicrobial tetracycline analogue A (Nitrocycline) designed using chemoinformatics | ||||
| International Journal of Health Sciences (Egypt) | ||||
| Articles in Press, Accepted Manuscript, Available Online from 24 July 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ijhegy.2023.213460.1029 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Mohammed M Kassab  
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| Instructor of microbiology,immunology,faculty of pharmacy,Cairo university,Egypt Specialist of pharmacology, toxicology and clinical pharmacy. | ||||
| Abstract | ||||
| Background In every country around the globe, bacterial resistance to antibiotics is a serious problem. Investigating new antibacterial medication sources is necessary in light of this. Tetracycline resistance is brought about through changes to the ribosome binding site and/or the development of mobile genetic elements carrying genes for resistance to tetracycline's. The objective of the study: The creation of new tetracycline antimicrobial analogues and testing of their in vitro antibacterial activity. Methodology: The current investigation was a screening experimental study. This research determined the minimum inhibitory concentrations (MI-Cs) of growth of several pathogenic microbes in Egypt by evaluating the in vitro antibacterial new tetracycline analog activity semi-synthetically synthesized from Streptomyces species. Tetracycline was purified using aqueous two-phase systems with polyethylene glycol and salts based on cholinium, which were then altered using chemo-informatics. The tetracycline analog A (nitrocycline) was created by adding an electron-withdrawing nitro group at position 7 of the tetracycline. Results: Although it showed less bacterial resistance than tetracycline, the antibiotic nitrocycline (tetracycline analogue A) was a more effective bacteriostatic antibacterial agent. When compared to the tetracycline prototype antibiotic, tetracycline analogue A demonstrated MICs of less than 10 micrograms/ml for bacterial growth, reflecting its potent antimicrobial activity. Conclusion: The development of a nitrocycline antibiotic with a broad range of activity, which helps in addressing the formidable problem of bacterial resistance, made this study intriguing. | ||||
| Keywords | ||||
| Tetracycline analog; design; antibiotics; screening; nitrocycline | ||||
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