Efficacy of Laser-Assisted Hatching on Pregnancy and Live-Birth Rates of Human Frozen-Thawed Embryos | ||||
| Journal of Scientific Research in Science | ||||
| Articles in Press, Corrected Proof, Available Online from 26 July 2023 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jsrs.2023.202776.1105 | ||||
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| Authors | ||||
Shorouk ElToukhy1; Mohamed Ali El-Desouky2; Ragaa Taha Mansour3; Mariam Abdur-Rahman   2
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| 1Chemistry, Faculty of Science, Cairo University, Cairo, Egypt | ||||
| 2Chemistry Department, Faculty of Science, Cairo University, Egypt | ||||
| 3The Egyptian IVF-ET Center, Cairo, Egypt | ||||
| Abstract | ||||
| Introduction: Unexplained repeated in-vitro fertilization (IVF) failure is a major problem that faces both genders. The present study aims to estimate the influence of the laser-assisted hatching (LAH) technique on implantation, clinical pregnancy and live-birth rates using human cryopreserved–thawed embryos produced by intracytoplasmic sperm injection (ICSI). Materials and methods: A total of 208 subjects was divided randomly into 100 subjects in the control group (Non-LAH), in which the uterine frozen embryo transfer (FET) was done directly; and 108 subjects in LAH group, in which laser shots were applied on day-3 frozen-thawed embryos before uterine transfer. All groups were further subdivided into three female age categories (22-30; 31-35 & 36-40 years). Number of mature, fertilized oocytes, fertilization, clinical pregnancy and live-birth rates were determined according to age distribution. Results: Both pregnancy (37.0 %) and live-birth (57.5 %) rates in LAH group were insignificantly lower than Non-LAH control group having pregnancy and live-birth rates of (45.0 % and 73.3 %, respectively). LAH caused a mild increase in clinical outcomes in females aged over 36 years, but in contrast, no improvement was observed in younger subjects. Conclusion: Our experimental study did not support using LAH as a routine strategy in frozen-thawed embryo transfer cycles. | ||||
| Keywords | ||||
| In-vitro fertilization; ICSI; Laser-assisted hatching; Frozen embryos; Pregnancy | ||||
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