Identification of inducible clindamycin resistance gene in Streptococcus agalactiae isolates colonizing pregnant women in Suez Canal University Hospitals

Abdullah, Omnia Mohamed; Elhadidy, Gehan Saddik; Khamiss, Rasha Elsayed; Taha, Omima Tharwat; Hamady, Asmaa Bakeir

doi:10.21608/mid.2023.213246.1531

	Identification of inducible clindamycin resistance gene in Streptococcus agalactiae isolates colonizing pregnant women in Suez Canal University Hospitals
Microbes and Infectious Diseases
Article 22, Volume 4, Issue 4, November 2023, Page 1246-1254 PDF (531.91 K)
Document Type: Original Article
DOI: 10.21608/mid.2023.213246.1531
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Authors
Omnia Mohamed Abdullah ¹; Gehan Saddik Elhadidy ²; Rasha Elsayed Khamiss³; Omima Tharwat Taha³; Asmaa Bakeir Hamady ²
¹Microbiology and Medical Immunology Department Faculty of Medicine Suez Canal University- Ismailia, Egypt
²Microbiology and Medical Immunology Department faculty of Medicine Suez Canal University - Ismailia
³Obstetrics and Gynaecology department faculty of Medicine Suez Canal University - Ismailia
Abstract
Background: Streptococcus agalactiae is classified as group B Streptococci (GBS) according to Lancefield classification, GBS resides as one of commensals of both genitourinary and gastrointestinal tracts of humans. GBS affects mainly immunocompromised people particularly neonates. An increased rates of macrolides and lincosamides resistance have been noted, which is associated with expression of erm genes, resulting in erythromycin and inducible clindamycin resistance. Aim: To limit Group B Streptococcus transmission to neonates and reduce neonatal morbidity and mortality rates in Suez Canal University Hospitals. Methods: 204 clinical vaginal swabs were collected from pregnant women admitted to obstetrics and gynaecology department in Suez Canal University hospitals. Swabs were incubated for 24 hours in lim’s selective broth and then for isolation on Colombia blood agar, then identification by colony morphology, Gram staining and biochemical reactions were done. Antimicrobial susceptibility testing of the isolates was performed to penicillin G, ampicillin, vancomycin, levofloxacin, cefotaxime, erythromycin and clindamycin by disk diffusion method and D test was then performed. conventional PCR was used to detect ermA gene. Results: Out of 204 specimens, 54 S. agalactiae isolates were isolated with prevalence of 26.47%. All S. agalactiae isolates were sensitive to penicillin G (100%). Out of the 33 erythromycin-resistant isolates, 5 isolates showed inducible phenotype, and 4 of them had ermA gene (80%). Conclusions: In this study, the prevalence of S. agalactiae was remarkable and antibiogram is mandatory to detect the inducible resistance phenotype. Further studies are needed to provide a comprehensive data about S. agalactiae sensitivity profile to antibiotics in other health care facilities to reduce neonatal morbidity and mortality.
Keywords
streptococcus agalactiae; Penicillin G; Clindamycin; Erythromycin; ermA


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