Production and Assessment of Antioxidant Activity of Exopolysaccharide from Marine Streptomyces globisporus BU2018
Abdel-Aziz, S., El awady, M., Nasr El-din, M., Almaraghy, H., Albahnasy, M. (2019). Production and Assessment of Antioxidant Activity of Exopolysaccharide from Marine Streptomyces globisporus BU2018. EKB Journal Management System, 59(3), 645-655. doi: 10.21608/ejbo.2019.6847.1274
Samir H. Abdel-Aziz; Mohamed El awady; Mohammed A. Nasr El-din; Heba Ibrahim Almaraghy; Mohamed E. Albahnasy. "Production and Assessment of Antioxidant Activity of Exopolysaccharide from Marine Streptomyces globisporus BU2018". EKB Journal Management System, 59, 3, 2019, 645-655. doi: 10.21608/ejbo.2019.6847.1274
Abdel-Aziz, S., El awady, M., Nasr El-din, M., Almaraghy, H., Albahnasy, M. (2019). 'Production and Assessment of Antioxidant Activity of Exopolysaccharide from Marine Streptomyces globisporus BU2018', EKB Journal Management System, 59(3), pp. 645-655. doi: 10.21608/ejbo.2019.6847.1274
Abdel-Aziz, S., El awady, M., Nasr El-din, M., Almaraghy, H., Albahnasy, M. Production and Assessment of Antioxidant Activity of Exopolysaccharide from Marine Streptomyces globisporus BU2018. EKB Journal Management System, 2019; 59(3): 645-655. doi: 10.21608/ejbo.2019.6847.1274

Production and Assessment of Antioxidant Activity of Exopolysaccharide from Marine Streptomyces globisporus BU2018

Egyptian Journal of Botany
Article 8, Volume 59, Issue 3, November 2019, Page 645-655  XML PDF (1.5 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2019.6847.1274
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Authors
Samir H. Abdel-Aziz1; Mohamed El awadyorcid 2; Mohammed A. Nasr El-din1; Heba Ibrahim Almaraghy3; Mohamed E. Albahnasy email 1
1Botany and Microbiology Department, Faculty of Science, Benha University, Benha, Egypt
2Department of Microbial Biotechnology, National Research Centre, Giza, Egypt
3Genetics Department, Faculty of Agriculture, Cairo University, Giza, Egypt
Abstract
MICROBIAL polysaccharides are involved in a wide range of biotechnological applications. Three streptomycetes isolates isolated from marine sediment from Sharm El-Sheikh were screened for their ability to produce exopolysaccharides (EPSs) as well as the antioxidant activity. Streptomyces globisporus BU2018 producing EPS which had the highest antioxidant activity was identified based on morphological, biochemical characteristics as well as molecular analysis of 16S rRNA gene. S. globisporus BU2018 produced 8.5g/L of EPS which was fractionated to give EPSSH3 as a major fraction and subjected to determine its antioxidant activities. Maximum antioxidant activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical was ~ 92.9±1.3% at 1500μg/ml after 120min and the half maximal inhibitory concentration (IC50) value was 500μg/ml after 60min. Therefore, the most extreme activity against H2O2 radical was 68.5±1.48% at 1500μg/ml after 60min with IC50 value of about 1000μg/ml after 60min. Moreover, the maximum reducing power activity of exopolysaccharide SH3 (EPSSH3) was at concentration 1000μg/ml and the most extreme metal chelating activity was 90.2% at 1000μg/ml. The EPSSH3 fraction contained uronic acid (65.13%) and no sulfate. The monosaccharide composition was glucuronic acid: galacturonic acid: rhamnose: fructose through molar ratio 4.0: 3.0: 1.0: 1.0, respectively. These indicate that the fraction is acidic heteropolysaccharide. The EPSSH3 had an overall average molecular weight (Mw) of 8.15×105g/mol and number average molecular weight (Mn) of 6.36×105g/mol. Otherwise, the fourier transform infrared spectrometry analysis proposed that it belongs to a β-type heteropolysaccharide.
Keywords
Exopolysaccharides; Streptomyces globisporus ; marine habitat; antioxidant
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