Carbapenemase Genes in Gram-Negative Bacteria: Detection and Implications in Clinical Isolates from Patient Samples
Egwuatu, T., Ogunrinde, O., Osibeluwo, B., Osuagwu, C. (2023). Carbapenemase Genes in Gram-Negative Bacteria: Detection and Implications in Clinical Isolates from Patient Samples. EKB Journal Management System, 15(2), 13-25. doi: 10.21608/eajbsg.2023.310649
Tenny O. G. Egwuatu; Oluwaseyi G. Ogunrinde; Bisola V. Osibeluwo; Chioma S. Osuagwu. "Carbapenemase Genes in Gram-Negative Bacteria: Detection and Implications in Clinical Isolates from Patient Samples". EKB Journal Management System, 15, 2, 2023, 13-25. doi: 10.21608/eajbsg.2023.310649
Egwuatu, T., Ogunrinde, O., Osibeluwo, B., Osuagwu, C. (2023). 'Carbapenemase Genes in Gram-Negative Bacteria: Detection and Implications in Clinical Isolates from Patient Samples', EKB Journal Management System, 15(2), pp. 13-25. doi: 10.21608/eajbsg.2023.310649
Egwuatu, T., Ogunrinde, O., Osibeluwo, B., Osuagwu, C. Carbapenemase Genes in Gram-Negative Bacteria: Detection and Implications in Clinical Isolates from Patient Samples. EKB Journal Management System, 2023; 15(2): 13-25. doi: 10.21608/eajbsg.2023.310649

Carbapenemase Genes in Gram-Negative Bacteria: Detection and Implications in Clinical Isolates from Patient Samples

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Volume 15, Issue 2, December 2023, Page 13-25  XML PDF (624.38 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2023.310649
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Authors
Tenny O. G. Egwuatu1; Oluwaseyi G. Ogunrinde1; Bisola V. Osibeluwo1; Chioma S. Osuagwu2
1Department of Microbiology, Faculty of Science, University of Lagos, Akoka, Nigeria
2Department of Medical Microbiology and Parasitology, Lagos University Teaching Hospital, Idi-Araba, Lagos, Nigeria.
Abstract
Background: Antimicrobial resistance is a great scourge on human health, exacerbated by the acquisition of resistance to carbapenems, the last resort treatment for infections caused by extended-spectrum beta-lactamase (ESBL) producing bacteria. Objectives: This cross-sectional study evaluated the prevalence of genes encoding ESBL and carbapenemase production in Gram-negative bacteria from clinical samples in Lagos state, Nigeria.Method: A total of 107 bacteria cultures were obtained from hospitals and clinical diagnostic laboratories. Isolate identification, antibiotics susceptibility testing, and phenotypic detection of ESBL production were done using standardized procedures. Multiplex polymerase chain reaction (PCR) was performed on ESBL-producing isolates to detect blaTEM, blaSHV, blaCTX-M, blaKPC, blaVIM, blaIMP and blaOXA.Result: Among the 107 cultures, 83 isolates were obtained with 55 being Gram-negative. Escherichia coli (22; 40%) was the most prevalent species followed by Klebsiella pneumoniae (7; 13%). Multidrug resistance (MDR) was observed in 34 (62%) of the isolated bacteria with 14 (26%) not susceptible to meropenem. ESBL production was detected in 42 (76%) of the isolates of which 23 (55%) strains harboured one or more of the genes blaTEM, blaSHV, and blaCTX-M. The carbapenemase genes blaKPC and/or blaVIM were observed in 11 (26%) isolates. No isolated bacteria were found to harbour blaIMP and/or blaOXA.Conclusion: Genes encoding ESBL and carbapenemase production were detected in samples of human origin in Lagos state. Novel antibiotics and/or alternative therapy are necessary for infection therapy in the near future.
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