IN VITRO ANTICANCER ACTIVITIES OF INK AND INTERNAL SHELL EXTRACTS OF SEPIA OFFICINALIS INHABITING EGYPTIAN WATER | ||||
| Bulletin of Faculty of Science, Zagazig University | ||||
| Article 13, Volume 2016, Issue 1, 2020, Page 160-174 PDF (767.14 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfszu.2016.31073 | ||||
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| Abstract | ||||
| The present study was carried out to assess the anticancer activity of extracts of internal shell and ink of S. officinalis on cell viability, MTT assay, cell cycle and apoptosis on Ehrlich Ascites Carcinoma (EAC) cells. The shell extracts have different anticancer effect on EAC cell in different concentrations (100, 250, 500, 1000 μg/ml) while ink extract has dose dependent effect according to following concentrations (100, 75, 50, 25 μg/ml). The viability of EAC exposed to sepia shell powder ranged from 23% (1000μg/ml) to 76% (100μg/ml) as well as the ink extracts shows cell viability ranged from 18% (100μg/ml) to 83% (25μg/ml). MTT assay showed also dose dependent manner of cell survival according to different concentrations of ink and shell extracts ranged from 61% (100μg/ml) to 100% (25μg/ml) of ink extract and from 61% (1000μg/ml) to 83% (100μg/ml) of sepia shell extract. EAC cells experienced a significant decrease in viability at low concentration with an eventual decline at the highest concentration compared to the reference drug cisplatin effect (40, 20μg/ml). The flow cytometry analysis of cell cycle and apoptosis of EAC cell treated with different concentrations of sepia shell and ink extracts of S. officinalis confirmed the previous results of viability and MTT assay sepia shell extracts. The obtained results lead to the conclusion that the cephalopod shell and ink are promising sources to be considered in the drug discovery for cancer therapy. | ||||
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