Effect of Topical application of Stem Cells - Conditioned Medium versus their derived Exosomes in Rat model of corneal Ulcer | ||||
| Journal of Medical Histology | ||||
| Article 1, Volume 6, Issue 1, June 2022, Page 1-15 PDF (2.13 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jmh.2023.187270.1112 | ||||
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| Authors | ||||
| Manar Ibrahim* 1; Soha Abdelwahab2; Sayed Ali3; Ahmed Mohamed4 | ||||
| 1Department of Histology and Cell Biology, Faculty of Medicine, Minia, University, Egypt. | ||||
| 2Department of Histology and Cell Biology, Faculty of Medicine, Minia, University, Egypt. | ||||
| 3Department o Anatomy, Faculty of Medicine, Minia, University, Egypt. | ||||
| 4Department of 1Histology and Cell Biology, Faculty of Medicine, Minia, University, Egypt. | ||||
| Abstract | ||||
| Background: Corneal ulcers are a serious vision frightening emergency. Even with accurate treatment, patients may suffer complications as perforation, scarring, cataracts, glaucoma, synechiae, and even loss of vision. Stem cells-based treatment is considered as a promising approach in corneal ulcer treatment. Now a days, alternative stem cell conditioned medium (CM) and exosomes (EXs) -based treatment for corneal ulcers are feasible approaches.. Aim: To compare the possible ameliorating effects of stem cells conditioned medium (CM) versus their derived exosomes (EXs) in corneal ulcer healing. Materials and Methods: 40 rats were randomly allocated into five groups; the control, corneal ulcer, CM-treated, EXs-treated and recovery groups; corneas were obtained for biochemical assessment of TNFα, VEGF, MDA and GSH levels and for histological structure . Corneal damage was assessed by histopathological scoring and immunohistochemical staining. Results: Corneal ulcer group revealed a significant increase in TNFα, VEGF and MDA levels and also revealed significant decrease in the GSH level. Histological assessment revealed structural alteration indicating severs corneal damage. Moreover a significant increase in P53 expression. Interestingly, CM and EXs administration significantly decreased TNFα, VEGF and MDA levels and increased GSH level. Additionally, there was obvious improvement in histological structure of corneas, and significant decrease in P53 expression. Conclusion: To the best of our knowledge, this is the first study to investigate CM versus EXs in treatment of corneal ulcer. Both EXs and CM ameliorate the healing of corneal ulcers with having a superior amelioration effect to EXs. The superior effect of EXs could be attributed to their properties as anti-apoptotic, anti-inflammatory, and anti-oxidative. | ||||
| Keywords | ||||
| Apoptosis; corneal ulcer; exosomes; TNFα; VEGF | ||||
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