The Possible Therapeutic Effect of Stem Cells-Conditioned Medium on Statin Induced Animal Model of Myopathy: Biochemical, Histological and Immuno-Histochemical Study | ||||
| Journal of Medical Histology | ||||
| Article 5, Volume 6, Issue 1, June 2022, Page 60-78 PDF (5.11 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jmh.2023.203584.1114 | ||||
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| Authors | ||||
| Manar Ibrahim* 1; Soha Abdelwahab1; Hager Mohammed,1; Amira Ahmed2 | ||||
| 1Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Egypt. | ||||
| 2Department of Histology and Cell Biology, Faculty of Medicine, Minia University, Egypt | ||||
| Abstract | ||||
| Introduction: Myopathies are diseases with high prevalence rate all over the world. This study aimed to investigate the ability of stem cell conditioned medium (SC-CM) administered by 2 different routes (IM\IV) to repair damaged skeletal muscle. Materials and Methods: This study included 60 adult male albino rats divided equally into five groups: the control group; the myopathy group in which myopathy was induced by administration of ATOR (10 mg/kg/day orally for 4 weeks); IV conditioned medium treated group in which myopathy was induced then the rats were IV injected with 0.5 ml of SC-CM in tail vein, IM conditioned medium treated group the rats were locally injected with 0.5 ml of SC-CM after induction of myopathy, the untreated myopathy group was left without treatment for 4 weeks. Blood samples and muscular specimens from all groups were collected for biochemical and histological studies. Results: The myopathy group showed marked muscle degeneration, with inflammatory cells infiltration. Compared with control, there was significant decrease in the area% of PAS and significant increase in mean creatinine kinase and Myoglobin levels , in area% of collagen and caspase3 positive reaction and the number of myogenin positive cells. Four weeks after SC-CM injection (IM\IV), there was marked improvement in histological architecture of the skeletal muscle as there was significant decrease in mean creatinine kinase and Myoglobin levels and in area% of collagen and caspase positive cells and significant increase in area% of PAS and number of myogenin positive cells and the improvement was more obvious in IV injected group. Conclusion: SC-CM injection may be of great help in the management of critical cases of myopathy especially by IV route. | ||||
| Keywords | ||||
| Conditioned medium; myopathy; statin | ||||
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