Bacteriological and Molecular studies on Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from chicken meat and its products in Kaliobia Governorate | ||||
| Benha Veterinary Medical Journal | ||||
| Article 11, Volume 31, Issue 1, September 2016, Page 64-72 PDF (407.94 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2016.31221 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ashraf A. Abd El Tawab1; Fatma I. El-Hofyand1; Ahmed A. A. Maarouf2; Dina H. Mousa3 | ||||
| 1Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Benha Univ. | ||||
| 2Animal Health Research "Benhabranch" | ||||
| 3veterinarian | ||||
| Abstract | ||||
| The study was performed on 175 random samples of fresh broiler chicken and chicken meat products viz: Chicken burger; chicken luncheon; chicken kofta and chicken sausage (35 for each), collected from different shops at Benha city, kaliobia government. The sample were examined for detection the prevalence of Staphylococci with special reference to S. aureus and MRSA, beside the phenotypic characterization of the isolated S. aureus strains and detection of their virulence genes in them. The bacteriological examination revealed the isolation of 98 Staphylococcus species including 41 S.aureus; 54 S. epidermidis and 3S. chromogenes. In addition, all 41 isolated S. aureus were coagulase positive strains, while, the other isolated Staphylococcal strains (57) were coagulase negative ones. Moreover, the results of SET- RPLA test revealed that 5 S. aureus strains out of 10 randomly examined strains were enterotoxigenic and classified according to type of toxin into (3A; 1 B & 1C). The sensitivity tests for the isolated S. aureus showed strains indicated high resistance to methicillin followed by oxacillin; Nalidixic acid; Ampicillin; Amoxicillin; Cefotaxime and Tobramycin, respectively. Meanwhile, they were highly sensitive to Gentamycin; Enrofloxacin; Norfloxacin; Lomefloxacin and Ciprofloxacin. PCR results cleared that, femA; mecA and hlb virulence genes were detected in all 5 S. aureus studied strains. Meanwhile, ica A was detected in 4studied strains; enterotoxin A (sea) was detected in 2 studied strains chicken meat and chicken kofta samples only and leukocidin (pvl) virulence gene was detected in 1 studied strain of chicken meat sample | ||||
| Keywords | ||||
| Chicken Meat Products; Bacteriological Evaluation; S. aureus; MRSA; PCR; Virulence factors | ||||
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