Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhea | ||
| Microbes and Infectious Diseases | ||
| Article 29, Volume 4, Issue 4, November 2023, Pages 1312-1324 PDF (822.15 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2023.222631.1563 | ||
| Authors | ||
| ragaa Sedeik Rashwan* 1; Shereen M. Galal2; Doaa M. Abd EL-kareem3; Aliaa M.A. Ghandour1; Rawhia F. Abd El-Hamid1 | ||
| 1Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt | ||
| 2Pediatrics, Gastroenterology, Hepatology, Faculty of Medicine, Assiut University Children Hospital | ||
| 3Department of Clinical Pathology, Faculty of Medicine, Assiut University Hospital | ||
| Abstract | ||
| Background: Faecal carriage of ESBL-producing bacteria is a potential risk for transmission and infection. Antibiotic resistance in diarrheal children is considered a risk factor for these young aged ones and limit treatment options. This study aimed to investigate the frequency of ESBL-producing Gram negative bacilli (GNB) phenotypically by Vitek and genotypically by PCR. Method: Seventy-three diarrheal isolates from outpatient children at of age range from less than 6 months to 18 months were isolated. Bacterial strains were identified and subjected to antimicrobial susceptibility testing by using the automated Vitek 2 compact system. All isolates were screened for ESBL phenotypically by Vitek and genotypically by PCR for blaCTXM, blaTEM and blaSHV. Results: Seventy three different GNB identified by Vitek system. The frequency of Escherichia coli was 68.5%, Acinetobacter haemolytica was 7 /73 (9.6%), Pseudomonas species (sp.) 4/73 (5.5%), Proteus mirabilis was (2.7%), Salmonella sp. was (1.4%), Enterobacter sp. was (6.8%), Aeromonas salmonicida was (1.4%), and Klebsiella was (4.1%). ESBL carriage was significantly high among isolated strains (64.4 %). High frequency of multidrug resistance (MDR) (90.4%) was found. High resistance was detected to ampicillin (98.6%), followed by cefazolin (93.2%) and ceftazidime (82.2%), trimethoprim-sulphamethoxazol (76.7%). Resistance to carbapenems was detected as (16.4%). blaCTX-M positive was (78.1%), blaTEM positive (53.4%), blaSHV positive (31.5%). Conclusions: The current study reported a high rate of faecal carriage of ESBL-producing and MDR GNB and children below 2 years of age. Resistance was observed to the available antimicrobials that are used for children in treatment. This leaves few treatment options for infections caused by these bacteria. | ||
| Keywords | ||
| Gram Negative Bacilli; ESBL; blaCTXM; blaTEM; blaSHV | ||
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