THE EFFECT OF FREEZE-DRYING ON THE KEEPING QUALITY OF ANTI-TETANIC SERUM
AHMED, F., SHAFEY, S., EL-SAYED, A., AWAD, M. (2002). THE EFFECT OF FREEZE-DRYING ON THE KEEPING QUALITY OF ANTI-TETANIC SERUM. EKB Journal Management System, 80(3), 1373-1381. doi: 10.21608/ejar.2002.312662
FAYKA K. AHMED; SAMY M. SHAFEY; AHMED A. EL-SAYED; MAGDY A. AWAD. "THE EFFECT OF FREEZE-DRYING ON THE KEEPING QUALITY OF ANTI-TETANIC SERUM". EKB Journal Management System, 80, 3, 2002, 1373-1381. doi: 10.21608/ejar.2002.312662
AHMED, F., SHAFEY, S., EL-SAYED, A., AWAD, M. (2002). 'THE EFFECT OF FREEZE-DRYING ON THE KEEPING QUALITY OF ANTI-TETANIC SERUM', EKB Journal Management System, 80(3), pp. 1373-1381. doi: 10.21608/ejar.2002.312662
AHMED, F., SHAFEY, S., EL-SAYED, A., AWAD, M. THE EFFECT OF FREEZE-DRYING ON THE KEEPING QUALITY OF ANTI-TETANIC SERUM. EKB Journal Management System, 2002; 80(3): 1373-1381. doi: 10.21608/ejar.2002.312662

THE EFFECT OF FREEZE-DRYING ON THE KEEPING QUALITY OF ANTI-TETANIC SERUM

Egyptian Journal of Agricultural Research
Article 30, Volume 80, Issue 3, September 2002, Page 1373-1381  XML PDF (2.08 MB)
Document Type: Original Article
DOI: 10.21608/ejar.2002.312662
View on SCiNiTO View on SCiNiTO
Authors
FAYKA K. AHMED1; SAMY M. SHAFEY2; AHMED A. EL-SAYED1; MAGDY A. AWAD1
1Veterinary Serum and Vaccine Research Institute, Agricultural Research Center, Ministry of Agriculture, Abbasia, Cairo, Egypt
2Central Laboratory of Control of Veterinary Biologics, Agricultural Research Centre, Ministry of Agriculture, Dokki, Giza, Egypt
Abstract
The purpose of this work is to obtain lyophilized preparation of tetanus antitoxin, and to study the effect of freeze-drying on the keep­ing quality of the lyophilized products. Six of antitetanic serum (ATS) products were prepared. These products were: liquid ATS with phenol 0.5%, lyophilized ATS with phenol 0.5%, lyophilized antitoxin immuno­globulin (ATI) with phenol 0.5%. liquid ATS with sodium azide 0.2%, lyo­philized ATS with sodium azide 0.2%, lyophilized ATI with 0.2% sodium azide. Saccharose 1% and glycine 2% were used as stabilizer during lyo­philized process. Sterility, safety and potency tests were applied for evaluation of different prepared lyophilized products. The results showed that the lyophilized products were easy re­constituted with distilled water. The products were prepared from MS with sodium azide 0.2%, ATS with phenol 0.5% and their concentrated antitoxin immunoglobulin (ATI) were bacteriology, mycoplasma and fun­gal sterile, potent and safe for injection. This study proved that the lyophilized process of the ATS either lyophilized as it is or lyophilized after concentration of antitoxin immuno­globulin has no effect on the sterility, safety and also has no significant effect on the potency of the freeze-dry products.
Statistics
Article View: 28
PDF Download: 112