The Impact of Staphylococcus aureus on Biofilm, Antibiotic Resistance and Cytotoxicity of Pseudomonas aeruginosa Isolated from Mixed Wound Infections | ||||
| Egyptian Journal of Medical Microbiology | ||||
| Volume 32, Issue 3, July 2023, Page 127-132 PDF (254.89 K) | ||||
| Document Type: New and original researches in the field of Microbiology. | ||||
| DOI: 10.21608/ejmm.2023.312957 | ||||
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| Authors | ||||
Areej M. El-Mahdy   1; Yasmen F. Mahran2
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| 1Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt | ||||
| 2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt | ||||
| Abstract | ||||
| Background: Pseudomonas aeruginosa and Staphylococcus aureus coexist in wounds causing severe illness. Objectives: This research was conducted to study the impact of S. aureus extract on P. aeruginosa. Methodology: Cell free extracts of four strains of S. aureus isolated from wounds were used to estimate their effect on biofilm formation, antibiotic resistance, and cytotoxicity of three clinical P. aeruginosa isolates from mixed wound infections. Results: Results disclosed that extracts of S. aureus significantly decreased biofilm formation and cytotoxicity in the tested P. aeruginosa isolates. Antibiotic resistance was assessed using three antibiotics; gentamicin, amikacin and imipenem. This is due to their higher resistance rates. Cell free extracts of S. aureus reduced antibiotic resistance of the tested P. aeruginosa isolates to aminoglycosides (Gentamicin and Amikacin) by (2-3) folds, while imipenem resistance was not changed.Conclusion: Based on this work's findings, S. aureus could affect P. aeruginosa in mixed wound infections through reduction of biofilm formation, cell cytotoxicity and antibiotic susceptibility. | ||||
| Keywords | ||||
| Wounds; Biofilm; Cytotoxic effect; Antibiotic susceptibilit | ||||
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