Biochemical Studies on Evaluation of New Composite against Hepatocellular carcinoma-induced in Rats: Recent Therapeutic Approaches | ||||
| Benha Veterinary Medical Journal | ||||
| Article 15, Volume 30, Issue 1, March 2016, Page 124-136 PDF (515.44 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2016.31357 | ||||
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| Authors | ||||
| Omaima Ragab abou Zaid1; Yakout A. El- Senousy1; Abdel Fattah M. Badawy2; Ahmed Mohammed Rashad3 | ||||
| 1Biochemistry Department, Faculty of Vet. Medicine, Benha University, Egypt | ||||
| 2Applied Chemistry Department, Egyptian Petroleum Research Institute, Egypt | ||||
| 3Biochemistry Department, Faculty of Pharmacy, October 6 University, Egypt | ||||
| Abstract | ||||
| The hepatoprotective effect of some natural and synthetic compounds against chemically induced hepatocellular carcinoma in rats was evaluated. One hundred male albino rats were divided equally into five groups. Normal control group, carcinogenic [Ferric nitrilotriacetate (Fe-NTA: 9 mg Fe/kg b.wt. i.p.) and chloroform (150 mg/ kg b.wt. orally)] - induced group, curcumin group (400 mg/kg. b.wt. orally), tetrachlorocuprate-lysine (25 mg/kg. b. wt. s.c.) and ascorbate (500 mg/kg. b. wt. orally) group and a mixture group (composed of curcumin, tetrachlorocuprate -lysine and ascorbate). Blood samples and liver tissue specimens were collected at the end of experiment (4 months) for determination of the following parameters: Tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), catalase and myeloperoxidase (MPO) in liver tissues, in addition to serum malondialdehyde (MDA) and reduced glutathione (GSH). Moreover, histopathological examination of liver tissues was done for results confirmation. The obtained results showed a significant elevation in MDA, MPO and immunological markers levels, with significant reduction in serum reduced glutathione and catalase activity in liver tissue in hepatocellular-carcinogen induced rats as compared to the control group. However, administration of rats with the compounds under investigation resulted in a significant reduction of MDA, MPO and immunological markers levels, and increased in reduced glutathione and catalase levels compared to the carcinogenic non treated group. Various pathological alterations were observed in liver of chemically induced-carcinogenic group interestingly, results supported the protective effect of the compounds under investigation and preserved the histological structures of liver tissues. These results concluded that basic curcumin, tetrachlorocuprate-lysine and ascorbate exert chemopreventative effect against hepatocellular carcinoma. | ||||
| Keywords | ||||
| Ferric nitrilotriacetate; Curcumin; antioxidant; hepatocellular carcinogen | ||||
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