Molecular Screening Of Virulence Genes In Avian Pathogenic Esherichia Coli | ||||
| Benha Veterinary Medical Journal | ||||
| Article 16, Volume 30, Issue 1, March 2016, Page 137-149 PDF (431.36 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2016.31358 | ||||
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| Authors | ||||
| Ashraf A. Abd El Tawab1; Fatma I. El-Hofy1; Ahmed M. Ammar2; Noha M. Abdel Galil3 | ||||
| 1Bacteriology, Immunology and Mycology Dept. Fac. Vet. Med., Benha Univ. | ||||
| 2Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Zagazig Univ. | ||||
| 3Animal Health Research Institute (Zagazig branch) | ||||
| Abstract | ||||
| Avian pathogenic Escherichia coli (APEC) causes colibacillosis, which is one of the main causes of economic losses in the poultry industry worldwide. This disease occurs only when the E. coli infecting strain presents virulence factors (encoded by specific genes) that enable the adhesion and proliferation in the host organism. Herin, 15 E. coli strains of different serogroups isolated from birds with colibacillosis were assigned to their phylogenetic groups and analyzed for the occurrence of 11 virulence associated genes. phylogenetic typing showed that group B2 was the largest (33.3%, 5/15) followed by group B1 (26.7%, 4/15), groups A and D were similar in size (22.1%, 19/86). The virulence profiles showed that ompA was found in most isolates 14/15 (93.3%). The iss gene was found in13/15 (86.6%). Followed by traT and iutA genes which were found in12/15 (80%). cvaC, stx2 and tsh genes were present in 9/15(60%), 7/15(46%) and of the isolates, respectively. Only one isolate gave positive amplification for stx1 and ibeA genes each. The hly gene was not encountered in any of the tested isolates | ||||
| Keywords | ||||
| Avian pathogenic Escherichia coli (APEC); Phylogenetic analysis; Virulence genes | ||||
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