Chemopreventive effect of omega- 3 fatty acids in N-nitrosodiethylamine Induced hepatocellular carcinoma in rats | ||||
| Benha Veterinary Medical Journal | ||||
| Article 17, Volume 30, Issue 1, March 2016, Page 150-160 PDF (589.2 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2016.31359 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Samy Hussein  1; Tahya E.A. Esmeal2; Aziza Amin3; Eman A.M. Sarhan4
| ||||
| 1Department of Biochemistry, Faculty of Vet. Med., Benha University, Egypt. | ||||
| 2Nutrition and clinical nutrition department, Faculty of Vet. Med., Benha University, Egypt | ||||
| 3Pathology Department, Faculty of Vet. Med., Benha University, Egypt | ||||
| 4Biochemistry Department, Faculty of Vet. Med., Benha University, Egypt | ||||
| Abstract | ||||
| Liver cancer, predominantly hepatocellular carcinoma (HCC), represents a complex and fatal malignancy driven primarily by oxidative stress and inflammation. The chemopreventive effect of omega-3 on inflammatory markers and oxidative damage, caspase-3, antioxidant status and histopathological alterations in hepatic tissue in N-nitrosodiethylamine (DEN)-induced HCC in male rats was investigated. To induce HCC, rats were given DEN (200 mg/kg b.wt.,i.p) three times at a 15 day interval. 75 rats were divided into five equal groups. Control group, DEN group, DEN + ω3 protected group received omega-3 (270mg/kg b. wt/day) orally 4 weeks before DEN injection and continued to 13 weeks, DEN + ω3 treated group firstly injected with DEN and orally treated with omega- 3 from the 8th week till the end of the experiment (13th week) and normal -ω3 group received omega-3. Blood samples and liver tissues were collected at 13th week. DEN-induced HCC significantly decreased SOD and CAT activities in liver tissue. However, a marked increase in liver tissue L-MDA, DNA fragmentation, caspase-3 and Nf-kB P65 and in serum AFP, IL-6 and TNF-α levels were observed in DEN injected rats. Initiation of HCC was demonstrated by histopathological examination of hepatic tissue in DEN injected group. Histopathological examination confirmed the pathological improvement in the liver of rats in omega -3 protected and treated groups. In conclusion, omega-3 demonstrates antiinflammatory property by suppression of TNF-alpha and IL-6 due to inhibition of NF-kB p65 and exerts its chemopreventive effect on liver carcinogenesis. These data suggest that omega-3 exhibited significant protection against DEN-induced HCC, which might be related with the enhancement of the antioxidant activity and the induction of apoptosis. | ||||
| Keywords | ||||
| Hepatocellular carcinoma; omega-3; Inflammatory mediators; oxidative damage; histopathology | ||||
|
Statistics Article View: 100 PDF Download: 379 |
||||
