A study of outer membrane protein (OMPs) genes for detection of salmonella organisms in poultry farms | ||||
Benha Veterinary Medical Journal | ||||
Article 25, Volume 30, Issue 1, March 2016, Page 231-237 PDF (325.16 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/bvmj.2016.31367 | ||||
View on SCiNiTO | ||||
Authors | ||||
Ashraf A. Abd El Tawab1; Mai M. Elsayed1; Ahmed M. Amaar2; Amany I. El-bialy3 | ||||
1Bacteriology, Immunology and Mycology Dep., Fac. Vet. Med. Banha Univ. | ||||
2Microbiology Dep., Fac. Vet. Med, Zagazig Univ. | ||||
3Serology Dep., animal health institute, Dokki | ||||
Abstract | ||||
Bacteriological examination of internal organs obtained from diseased layers, broilers and baby chicks revealed isolation of salmonellae with an incidence 5%, 2.5% and 7.1%, respectively. Eleven Salmonella isolates were recovered out of 250 poultry samples. The highest percentage rate was in liver 4%, followed by intestine 2.4% and finally spleen 0.8%. Salmonella organisms were recovered from ovary of layers with an incidence 3.33%. Serological identification of Salmonella isolates revealed that the most prevalent serovars was. Enteritidis (3) followed by. Gallinarum (2) and Rissen (2) then S. Kentukey, S. Florida, S. Lomita and S. Sontheim (one isolate for each). The goal of this study was to evaluate the suitability of the outer membrane genes OmpA and OmpF for detection of Genus Salmonella in clinical samples. OmpA gene was detected in 100% of the examined clinical samples while OmpF gene was detected in 14 clinical samples. Three samples were positive by OmpF.PCR more than cultural method. It was concluded that PCR protocol decrease the time needed for the detection of Salmonella OmpA gene couldn't discriminate genus Salmonella from other non-Salmonella organisms in clinical samples .AS it amplified OmpA in other Enterobacteriaceae. However, OmpF gene is promising tool for detection of Genus Salmonella | ||||
Keywords | ||||
S. enteritidis; outer membrane protein genes; PCR | ||||
Statistics Article View: 114 PDF Download: 131 |
||||